Gustavo Gabriel Mo Munoz, Brix, Oliver , Kolocke, Philipp , Wendler, Nicolas Dario, Lerner, Eitan , Zijlstra, Niels , ו Cordes, Thorben . Submitted.
“Single-Molecule Detection And Super-Resolution Imaging With A Portable And Adaptable 3D-Printed Microscopy Platform (Brick-Mic)”. Biorxiv. .
Publisher's Version תקציר Over the past decades, single-molecule spectroscopy and super-resolution microscopy have advanced significantly and by now represent important tools for life science research. Despite rapid progress and ongoing development, there is a growing gap between the state-of-the-art and what is accessible to non-optics specialists, e.g., biologists, biochemists, medical researchers, and labs with financial constraints. To bridge this gap, we introduce Brick-MIC, a versatile and affordable open-source 3D-printed micro-spectroscopy and imaging platform. Brick-MIC enables the integration of various fluorescence imaging techniques with single-molecule resolution within a single platform and enables exchange between different modalities within minutes. In this work, we present three variants of Brick-MIC that facilitate single-molecule fluorescence detection, fluorescence correlation spectroscopy and super-resolution imaging. With the three variants, we were able to observe conformational changes and absolute inter-dye distances in single macromolecules and perform single-molecule localization microscopy (STORM and PAINT) of DNA origami nanostructures. Detailed descriptions of the hardware and software components, as well as data analysis routines are provided, to allow non-optics specialist to operate their own Brick-MIC with minimal effort and investments. We foresee that our affordable, flexible, and open-source Brick-MIC platform will be a valuable tool for many laboratories worldwide.
Neta Kligler-Vilenchik, Tenenboim-Weinblatt, Keren , Boczkowski, Pablo J. , Hayashi, Kaori , Mitchelstein, Eugenia , ו Villi, Mikko . Submitted.
“Youth Political Talk In The Changing Media Environment: A Cross-National Typology”. The International Journal Of Press/Politics.
תקציר While political communication scholarship has long underscored the importance of political talk—casual conversations about news and politics that occur in everyday situations—as a way for citizens to clarify their opinions and as a precursor for political engagement, much of this literature tends to depict political talk as uncomfortable and difficult for citizens. Yet, this focus on the challenging aspects of political talk has been informed predominantly by the US context. To what extent may a different picture emerge when looking across different cultural contexts? And how are these dynamics shaped by the affordances of the multi-platform social media environment? This paper explores these questions through a unique dataset of 122 qualitative interviews conducted between 2016 and 2019 with young people (ages 18–29) from five countries: Argentina, Finland, Israel, Japan, and the United States. Rather than solidifying the avoidance of controversial political talk as the key strategy at the disposal of young people, our findings point at a five-pronged typology of young people, with each type representing a different approach toward political talk. Our typology thus contributes to a more comprehensive and nuanced picture of various approaches towards political talk employed by young people across different countries and in relation to different digital media affordances.
