פרסומים

Mainstream virus detection relies on the specific amplification of nucleic acids via polymerase chain reaction, a process that is slow and requires extensive laboratory expertise and equipment. Other modalities, such as antigen-based tests, allow much faster virus detection but have reduced sensitivity. In this study, we report the development of a flow virometer for the specific and rapid detection of single nanoparticles based on confocal microscopy. The combination of laminar flow and multiple dyes enable the detection of correlated fluorescence signals, providing information on nanoparticle volumes and specific chemical composition properties, such as viral envelope proteins. We evaluated and validated the assay using fluorescent beads and viruses, including SARS-CoV-2. Additionally, we demonstrate how hydrodynamic focusing enhances the assay sensitivity for detecting clinically-relevant virus loads. Based on our results, we envision the use of this technology for clinically relevant bio-nanoparticles, supported by the implementation of the assay in a portable and user-friendly setup.

Aerosol-cloud interactions are a persistent source of uncertainty in climate research. This study presents findings from a model intercomparison project examining the impact of aerosols on clouds and climate in convection permitting Radiative-Convective Equilibrium (RCE) simulations. Specifically, 11 different modeling teams conducted RCE simulations under varying aerosol concentrations, domain configurations, and sea surface temperatures (SSTs). We analyze the response of domain-mean cloud and radiative properties to imposed aerosol concentrations across different SSTs. Additionally, we explore the potential impact of aerosols on convective aggregation and large-scale circulation in large-domain simulations.

The results reveal that the cloud and radiative responses to aerosols vary substantially across models. However, a common trend across models, SSTs, and domain configurations is that increased aerosol loading tends to suppress warm rain formation, enhance cloud water content in the mid-troposphere, and consequently increase mid-tropospheric humidity and upper-tropospheric temperature, thereby impacting static stability. The warming of the upper troposphere can be attributed to reduced lateral entrainment effects due to the higher environmental humidity in the mid-troposphere. However, models do not agree on aerosol impacts on convective updraft velocity based on the preliminary examination of high-percentiles of vertical velocity at a single mid-troposheric layer (500hPa). In large-domain simulations, where convection tends to self-organize, aerosol loading does not consistently influence self-organization but tends to reduce the intensity of large-scale circulation forming between convective clusters and dry regions. This reduction in circulation intensity can be explained by the increase in static stability due to the upper tropospheric warming. 

Over the past decades, single-molecule spectroscopy and super-resolution microscopy have advanced significantly and by now represent important tools for life science research. Despite rapid progress and ongoing development, there is a growing gap between the state-of-the-art and what is accessible to non-optics specialists, e.g., biologists, biochemists, medical researchers, and labs with financial constraints. To bridge this gap, we introduce Brick-MIC, a versatile and affordable open-source 3D-printed micro-spectroscopy and imaging platform. Brick-MIC enables the integration of various fluorescence imaging techniques with single-molecule resolution within a single platform and enables exchange between different modalities within minutes. In this work, we present three variants of Brick-MIC that facilitate single-molecule fluorescence detection, fluorescence correlation spectroscopy and super-resolution imaging. With the three variants, we were able to observe conformational changes and absolute inter-dye distances in single macromolecules and perform single-molecule localization microscopy (STORM and PAINT) of DNA origami nanostructures. Detailed descriptions of the hardware and software components, as well as data analysis routines are provided, to allow non-optics specialist to operate their own Brick-MIC with minimal effort and investments. We foresee that our affordable, flexible, and open-source Brick-MIC platform will be a valuable tool for many laboratories worldwide.