פרסומים

This paper presents a KGW Raman laser with an external-cavity configuration at the 2 &\#x00B5;m region. The Raman laser is pumped by an actively Q-switched Tm:YLF laser, especially designed for this purpose emitting at 1880 nm. Due to the KGW bi-axial properties, the Raman laser is able to lase separately at two different output lines, 2197 nm and 2263 nm. The output energies and pulse durations that were achieved for these two lines are 0.15 mJ/pulse at 21 ns and 0.4 mJ/pulse at 5.4 ns, respectively. To the best of our knowledge, this is the first time that the KGW crystal, which is well known for its wide use in shorter wavelengths, is demonstrated in a Raman laser in the 2 &\#x00B5;m region. According to the achieved results and due to the KGW properties, it appears to be a suitable crystal for energy scaling and efficient Raman conversion in this spectral range. An estimation of the Raman gain coefficient for this wavelength is provided as well.

Since its emergence around 12,000 years ago, agriculture has transformed our species, other species, and the planet on which we all live. Here we argue that the emergence and impact of agriculture can be understood within new theoretical frameworks developing within the evolutionary human sciences. First, the improvement and diversification of agricultural knowledge, practices, and technology is a case of cumulative cultural evolution, with successive modifications accumulated over multiple generations to exceed what any single person could create alone. We discuss how the factors that permit, facilitate, and hinder cumulative cultural evolution might apply to agriculture. Second, agriculture is a prime example of gene-culture co-evolution, where culturally transmitted agricultural practices generate novel selection pressures for genetic evolution. While this point has traditionally been made for the human genome, we expand the concept to include genetic changes in domesticated plants and animals, both via traditional breeding and molecular breeding. Third, agriculture is a powerful niche-constructing activity that has extensively transformed the abiotic, biotic, and social environments. We examine how agricultural knowledge and practice shapes, and are shaped by, social norms and attitudes. We discuss recent biotechnology and associated molecular breeding techniques and present several case studies, including golden rice and stress resistance. Overall, we propose new insights into the co-evolution of human culture and plant genes and the unprecedented contribution of agricultural activities to the construction of unique agriculture-driven anthropogenic biomes. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

We map a class of well-mixed stochastic models of biochemical feedback in steady state to the mean-field Ising model near the critical point. The mapping provides an effective temperature, magnetic field, order parameter, and heat capacity that can be extracted from biological data without fitting or knowledge of the underlying molecular details. We demonstrate this procedure on fluorescence data from mouse T cells, which reveals distinctions between how the cells respond to different drugs. We also show that the heat capacity allows inference of the absolute molecule number from fluorescence intensity. We explain this result in terms of the underlying fluctuations, and we demonstrate the generality of our work.

Infection by large dsDNA viruses can lead to a profound alteration of host transcriptome and metabolome in order to provide essential building blocks to support the high metabolic demand for viral assembly and egress. Host response to viral infection can typically lead to diverse phenotypic outcome that include shift in host life cycle and activation of anti-viral defense response. Nevertheless, there is a major bottleneck to discern between viral hijacking strategies and host defense responses when averaging bulk population response. Here we study the interaction between Emiliania huxleyi, a bloom-forming alga, and its specific virus (EhV), an ecologically important host-virus model system in the ocean. We quantified host and virus gene expression on a single-cell resolution during the course of infection, using automatic microfluidic setup that captures individual algal cells and multiplex quantitate PCR. We revealed high heterogeneity in viral gene expression among individual cells. Simultaneous measurements of expression profiles of host and virus genes at a single-cell level allowed mapping of infected cells into newly defined infection states and allowed detection specific host response in a subpopulation of infected cell which otherwise masked by the majority of the infected population. Intriguingly, resistant cells emerged during viral infection, showed unique expression profiles of metabolic genes which can provide the basis for discerning between viral resistant and susceptible cells within heterogeneous populations in the marine environment. We propose that resolving host-virus arms race at a single-cell level will provide important mechanistic insights into viral life cycles and will uncover host defense strategies. © 2019 Rosenwasser et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Acute kidney injury (AKI) in cats is associated with high mortality, partially attributed to late recognition of the disease when using currently available markers. Feline chronic kidney disease (CKD) has a variable progression rate. This study aimed to evaluate the sensitivity and specificity of urinary heat shock protein-72 to urinary creatinine ratio (uHSP72:uCr) as a diagnostic and prognostic marker in feline AKI, and as a prognostic indicator in feline CKD. The study included 63 cats, divided into five groups: healthy controls (n=10), urethral obstruction (UO; n=7), CKD (n=15), AKI (16 cats) and acute decompensating CKD (ACKD; n=15). Median uHSP72:uCr (ng/mg) of healthy, UO, CKD, AKI and ACKD cats were 0.44 (range, 0.13–1.1), 1.96 (range, 0.64–11.9), 4.2ng/mg (range, 0.57–22.16), 3.2 (range, 0.42–10.91) and 7.0 (range, 1.2–20.96), respectively, and differed (P<0.001) among groups. uHSP72:uCr was significantly lower in the controls vs. the CKD, AKI and ACKD groups. Receiver operator characteristic analysis of uHSP72:uCr, including the AKI and control groups, showed an area under the curve of 0.93 (95% confidence interval, 0.84–1.00), indicating an excellent predictive performance for diagnosing AKI. A 0.54ng/mg cutoff point corresponded to 94% sensitivity and 70% specificity for diagnosing AKI. The median survival time of cats with CKD with low uHSP72:uCr was longer (P=0.036) than in those with high uHSP72:uCr (561 vs. 112 days, respectively). uHSP72:uCr is a highly sensitive, moderately specific marker of AKI in cats, and is associated with the survival of cats with CKD.