We demonstrate a directional beaming of photons emitted from nanocrystal quantum dots that are embedded in a subwavelength metallic nanoslit array with a divergence angle of less than 4°. We show that the eigenmodes of the structure result in localized electromagnetic field enhancements at the Bragg cavity resonances, which could be controlled and engineered in both real and momentum space. The photon beaming is achieved using the enhanced resonant coupling of the quantum dots to these Bragg cavity modes, which dominates the emission properties of the quantum dots. We show that the emission probability of a quantum dot into the narrow angular mode is 20 times larger than the emission probability to all other modes. Engineering nanocrystal quantum dots with subwavelength metallic nanostructures is a promising way for a range of new types of active optical devices, where spatial control of the optical properties of nanoemitters is essential, on both the single and many photons level.

Butyrylcholinesterase (BChE) is the major acetylcholine hydrolyzing enzyme in peripheral mammalian systems. It can either reside in the circulation or adhere to cells and tissues and protect them from anticholinesterases, including insecticides and poisonous nerve gases. In humans, impaired cholinesterase functioning is causally involved in many pathologies, including Alzheimer’s and Parkinson’s diseases, trait anxiety, and post stroke conditions. Recombinant cholinesterases have been developed for therapeutic use; therefore, it is important to follow their in vivo path, location, and interactions. Traditional labeling methods, such as fluorescent dyes and proteins, generally suffer from sensitivity to environmental conditions, from proximity to different molecules or special enzymes which can alter them, and from relatively fast photobleaching. In contrast, emerging development in synthesis and surface engineering of semiconductor nanocrystals enable their use to detect and follow molecules in biological milieus at high sensitivity and in real time. Therefore, we developed a platform for conjugating highly purified recombinant human BChE dimers (rhBChE) to CdSe/CdZnS quantum dots (QDs). We report the development and characterization of highly fluorescent aqueous soluble QD-rhBChE conjugates, present maintenance of hydrolytic activity, inhibitor sensitivity, and adherence to the membrane of cultured live cells of these conjugates, and outline their advantageous features for diverse biological applications.

Doping of semiconductors by impurity atoms enabled their widespread technological application in microelectronics and optoelectronics. However, doping has proven elusive for strongly confined colloidal semiconductor nanocrystals because of the synthetic challenge of how to introduce single impurities, as well as a lack of fundamental understanding of this heavily doped limit under strong quantum confinement. We developed a method to dope semiconductor nanocrystals with metal impurities, enabling control of the band gap and Fermi energy. A combination of optical measurements, scanning tunneling spectroscopy, and theory revealed the emergence of a confined impurity band and band-tailing. Our method yields n- and p-doped semiconductor nanocrystals, which have potential applications in solar cells, thin-film transistors, and optoelectronic devices.

Provided is a core/multishell semiconductor nanocrystal including a core and multiple shells, which exhibits a type-I band offset and high photoluminescence quantum yield providing a bright tunable emission covering the visible range from about 400 nm to NIR over 1600 nm.