פרסומים

Abstract Olive (Olea europaea L.) inflorescences, formed in lateral buds, flower in spring. However, there is some debate regarding time of flower induction and inflorescence initiation. Olive juvenility and seasonality of flowering were altered by overexpressing genes encoding flowering locus T (FT). OeFT1 and OeFT2 caused early flowering under short days when expressed in Arabidopsis. Expression of OeFT1/2 in olive leaves and OeFT2 in buds increased in winter, while initiation of inflorescences occurred i n late winter. Trees exposed to an artificial warm winter expressed low levels of OeFT1/2 in leaves and did not flower. Olive flower induction thus seems to be mediated by an increase in FT levels in response to cold winters. Olive flowering is dependent on additional internal factors. It was severely reduced in trees that carried a heavy fruit load the previous season (harvested in November) and in trees without fruit to which cold temperatures were artificially applied in summer. Expression analysis suggested that these internal factors work either by reducing the increase in OeFT1/2 expression or through putative flowering repressors such as TFL1. With expected warmer winters, future consumption of olive oil, as part of a healthy Mediterranean diet, should benefit from better understanding these factors.

We suggest two psychological mechanisms, temporal discounting and feeling of resource scarcity, for explaining the relation between food insecurity and body overweight. We demonstrate how Nettle et al.'s findings could be explained, post hoc, by each of these accounts, suggesting that their data are not rich enough to allow identification of mechanisms that underlie food insecurity and overweight relationship.

COT1 is the founding member of the highly conserved nuclear Dbf2-related (NDR) Ser/Thr kinase family and plays a role in the regulation of polar growth and development in and other fungi. Changes in COT1 phosphorylation state have been shown to affect hyphal elongation, branching, and conidiation. The function of NDR protein kinases has been shown to be regulated by type 2A protein phosphatases (PP2As). PP2As are heterotrimers comprised of a catalytic and scaffolding protein along with an interchangeable regulatory subunit involved in determining substrate specificity. Inactivation of the PP2A regulatory subunits and conferred severe hyphal growth defects. Partial suppression of defects observed in the strain (but not in the Δ mutant) was observed in phosphomimetic mutants, demonstrating that altering COT1 phosphorylation state can bypass, at least in part, the requirement of a functional RGB1 subunit. The functional fusion proteins RGB1::GFP and B56::GFP predominantly localized to hyphal tips and septa, respectively, indicating that their primary activity is in different cellular locations. COT1 protein forms exhibited a hyperphosphorylated gel migration pattern in an mutant background, similar to that observed when the fungus was cultured in the presence of the PP2A inhibitor cantharidin. COT1 was hypophosphorylated in a Δ mutant background, suggesting that this regulatory subunit may be involved in determining COT1 phosphorylation state, yet in an indirect manner. Reciprocal co-immunoprecipitation analyses, using tagged COT1, PPH1, RGB1, and B56 subunits established that these proteins physically interact. Taken together, our data determine the presence of a functional and physical link between PP2A and COT1 and show that two of the PP2A regulatory subunits interact with the kinase and determine COT1 phosphorylation state.

We present experimental and numerical studies on principal modes in a multimode fiber with mode coupling. By applying external stress to the fiber and gradually adjusting the stress, we have realized a transition from weak to strong mode coupling, which corresponds to the transition from single scattering to multiple scattering in mode space. Our experiments show that principal modes have distinct spatial and spectral characteristic in the weak and strong mode coupling regimes. We also investigate the bandwidth of the principal modes, in particular, the dependence of the bandwidth on the delay time, and the effects of the mode-dependent loss. By analyzing the path-length distributions, we discover two distinct mechanisms that are responsible for the bandwidth of principal modes in weak and strong mode coupling regimes. Their interplay leads to a non-monotonic transition of the average principal mode bandwidth from weak to strong mode coupling. Taking into account the mode-dependent loss in the fiber, our numerical results are in qualitative agreement with our experimental observations. Our study paves the way for exploring potential applications of principal modes in communication, imaging and spectroscopy.

Objective Examine physicians’ implementation of effective communication principles with patients with intellectual disabilities (ID) and its predictors. Methods Focus groups helped construct a quantitative questionnaire. The questionnaire (completed by 440 physicians) examined utilization of effective communication principles, attitudes toward individuals with ID, subjective knowledge and number of patients with ID. Results Subjective knowledge of ID and more patients with ID increased utilization of effective communication principles. Provision of knowledge that allows patients to make their own medical decisions was predicted by more patients with ID, lower attitudes that treatment of this population group is not desirable, less negative affect and greater perception that treatment of this group is part of the physician's role. Effective preparation of patients with ID for treatment was predicted by higher perception of treatment of this group as part of the physician's role, lower perception of this field as undesirable and higher perception of these individuals as unable to make their own choice. Simplification of information was predicted by a greater perception of treatment of this group as part of the physician's role and more negative affect. Conclusion Greater familiarity may enhance care for these patients. Practice implications Increase exposure to patients with ID within training.