Does philosophy gain or lose when it is embedded within literature or embodied by drama? Does literary criticism gain or lose when it turns to literary works as occasions for abstract reflection? Leading literary scholars and philosophers interrogate philosophical dimensions of Shakespeare's Hamlet with these urgent questions in view. Scholars probe Hamlet's own insights, assess the significance of philosophy's literary-dramatic framing by this play, and trace the philosophically-relevant underpinnings revealed by historical transformations in Hamlet's reception. They focus on the play's thematizations of subjectivity, knowledge, sex, grief, self-theatricalization. Examining Shakespeare's play from a philosophical standpoint sharpens the questions the play itself so famously poses: What counts as a proper response to injustice upon realizing that whatever one does, there can be no undoing of the initial wrong? What do our commitments to the dead amount to? How to persist in infusing significance into action while grasping the degradation of death and our own replaceability? Scholars at the forefront of their fields tackle these and other questions from a wide range of viewpoints, illuminating the central concerns of one of Shakespeare's masterpieces.
This book examines the transition from traditional to modern elegy through a close study of Thomas Hardy’s oeuvre and its commitment to mourning and remembrance. Hardy is usually read as an avowed elegist who writes against the collective forgetfulness typical of the late-Victorian era. But Hardy, as argued here, is dialectically implicated in the very cultural and psychological amnesia that he resists, as her book demonstrates by expanding the corpus of study beyond the spousal elegies (the “Poems of 1912-1913”) to include a wide variety of poems, novels and short stories that deal with bereavement and mourning. Locating the modern aspect of Hardy’s elegiac writing in this ambivalence and in the subversion of memory as unreliable, the book explores the textual moments at which Hardy challenges binary dichotomies such as forgetting vs. remembering, narcissism vs. unselfish commitment, grief vs. betrayal, the work of mourning vs. melancholia, presence vs. absence. The book's analysis allows us to relate Hardy’s elegiac poetics, and particularly his description of the mourner as a writer, to shifting late-Victorian conceptualizations of death, memory, art, science and gender relations.
Argumentation has been recognized as an important classroom activity and as a potentially powerful means for learning complex academic content. However, eliciting and sustaining student-to-student argumentive discourse that is both critical as well as constructive is also known to be notoriously difficult. Whereas previous research has traditionally focused on the cognitive, meta-cognitive and task-related antecedents and conditions for productive student argumentation, in the present work we explore two social-motivational factors that may provide insight into this difficulty, namely students’ individual achievement goals and gender. In two separate studies, undergraduate students indicated their intentions to engage in different discourse types when asked to discuss their solutions to a complex topic from astronomy (N = 245, Study 1) or economics (N = 98, Study 2) with a disagreeing peer. In addition to the productive, ideal type of argumentive discourse for learning purposes (i.e., deliberative argumentation), three additional discourse types were targeted that typically ensue, but are considered less productive (i.e., disputative argumentation, quick consensus seeking and private deliberation). The overall pattern of results show that mastery goals (a focus on developing competence and task mastery) are associated with deliberative argumentation and with private deliberation. In contrast, performance-approach goals (a focus on demonstrating competence relative to others) as well as high confidence are associated with disputative peer argumentation. Quick consensus seeking was predicted by higher performance-avoidance goals (a focus on avoiding incompetence relative to others) and lower mastery goals. No consistent gender differences were found. Taken together, the results extend previous work in socio-cognitive conflict settings and emphasize the role of achievement goals in peer argumentation.
AbstractProbiotics, live microbial supplements, are often incorporated into foods and beverages to provide putative health benefits. To ensure their beneficial effects, these organisms must survive processing and storage of food, its passage through the upper gastrointestinal tract (GIT), and subsequent chemical ingestion processes until they reach their target organ. However, there is considerable loss of viability of probiotic bacteria in the acidic conditions of the stomach and the high bile concentration in the small intestine. Bacillus subtilis, a spore-forming non-pathogenic bacterium, recently has gained interest in its probiotic properties; it can effectively maintain a favorable balance of microflora in the GIT. In addition, B. subtilis produces an extracellular matrix that protects it from stressful environments. We suggested that the extracellular matrix produced by B. subtilis could protect other probiotic bacteria and therefore potentially could be used as a vehicle for delivering viable probiotic cells to humans. Therefore, we developed a novel cultivation system that enables co-culturing of B. subtilis along with probiotic lactic acid bacteria (LAB) by increasing production of the extracellular matrix by B. subtilis cells. Moreover, we showed that B. subtilis improved survivability of LAB during food preparation, storage and ingestion. Therefore, we believe that the results of our study will provide a novel technique of using a natural system for preservation and delivery of probiotics to humans.
Plasmids harboring qnr genes confer resistance to low fluoroquinolone concentrations. These genes are of significant clinical, evolutionary and environmental importance, since they are widely distributed in a diverse array of natural and clinical environments. We previously extracted and sequenced a large (∼185 Kbp) qnrB-harboring plasmid, and several small (∼8 Kbp) qnrS-harboring plasmids, from Klebsiella pneumoniae isolates from municipal wastewater biosolids, and hypothesized that these plasmids provide host bacteria a selective advantage in wastewater treatment plants (WWTPs) that often contain residual concentrations of fluoroquinolones. The objectives of this study were therefore to determine the effect of residual fluoroquinolone concentrations on the growth kinetics of qnr plasmid-harboring bacteria; and on the copy number of qnr plasmids and expression of qnr genes. Electrotransformants harboring either one of the two types of plasmids could grow at ciprofloxacin concentrations exceeding 0.5 μg ml(-1), but growth was significantly decreased at concentrations higher than 0.1 μg ml(-1). In contrast, plasmid-free strains failed to grow even at 0.05 μg ml(-1). No differences were observed in plasmid copy number under the tested ciprofloxacin concentrations, but qnr expression increased incrementally from 0 to 0.4 μg ml(-1), suggesting that the transcription of this gene is regulated by antibiotic concentration. This study reveals that wastewater-derived qnr plasmids confer a selective advantage in the presence of residual fluoroquinolone concentrations and provides a mechanistic explanation for this phenomenon.
