An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells

Citation:

I. Lavi, Friesem, D. , Geresh, S. , Hadar, Y. , and Schwartz, B. . 2006. “An Aqueous Polysaccharide Extract From The Edible Mushroom Pleurotus Ostreatus Induces Anti-Proliferative And Pro-Apoptotic Effects On Ht-29 Colon Cancer Cells”. Cancer Lett, 244, 1, Pp. 61-70. doi:10.1016/j.canlet.2005.12.007.

Abstract:

Anti-proliferative and pro-apoptotic activities of fractions of Pleurotus ostreatus were examined using HT-29 colon cancer cells in vitro. A hot-water-soluble fraction of the mycelium of the liquid cultured mushroom was partially isolated and chemically characterized as a low-molecular-weight alpha-glucan. HT-29 cells were exposed to the different isolates and significant inhibition of proliferation was obtained in a dose-dependent manner. Proliferation inhibition was shown to be the result of apoptotic induction because the pro-apoptotic molecules Bax and cytosolic cytochrome-c were upregulated. Fluorescence-activated cell sorter analyses of polysaccharide-treated HT-29 cells showed a high percentage of Annexin-positive cells. Here, we describe a newly identified low-molecular-weight alpha-glucan with promising anti-tumorigenic properties, and demonstrate its direct effect on colon cancer cell proliferation via induction of programmed cell death.

Notes:

Lavi, Iris Friesem, Dana Geresh, Shimona Hadar, Yitzhak Schwartz, Betty eng Research Support, Non-U.S. Gov't Ireland Cancer Lett. 2006 Nov 28;244(1):61-70. doi: 10.1016/j.canlet.2005.12.007. Epub 2006 Jan 18.