Existing explanations of systematic undersupply of policy (e.g., institutional frictions, policy drift, and loss aversion) highlight the role of institutional and cognitive factors in the policy process while paying little attention to the role of emotions and emotional sentiments (e.g., policy mood). To bridge this gap, this article conceptualizes the role of negative emotions (e.g., fear, anger, hatred, disgust) and emotional sentiments in driving systematic policy underreaction (or what I have termed a negative policy bubble). Regarding the birth of emotion-driven negative policy bubbles, the behavioral understanding advanced here points to (1) an endogenous process that affects opinion formation, attention, learning, behavior, and attitudes; (2) an exogenous shock that “turns on” an endogenous process; (3) emotional manipulation by emotional entrepreneurs, or (4) a process by which the psychological context within which the policy process takes place conditions policy dynamics. Self-reinforcing processes interact with the contagion of emotions, imitation, and herd behavior to reinforce the lack of confidence in the policy, thereby creating a lock-in effect of systematic undersupply of policy. This process may be interrupted following modest endogenous or exogenous perturbations; a decrease in the intensity and duration of negative emotions and/or an increase in their speed of decline by emotional entrepreneurs, as well as following the reduction in negativity bias when the information environment becomes predominantly negative. The paper also provides guidance on productive directions for future research.
OBJECTIVES: Bipolar disorder (BD) is a complex psychiatric disorder characterized by mania and depression. Alterations in brain Na(+) , K(+) -ATPase and cardiac steroids (CSs) have been detected in BD, raising the hypothesis of their involvement in this pathology. The present study investigated the behavioral and biochemical consequences of a reduction in endogenous brain CS activity in animal models of mania. METHODS: Amphetamine (AMPH)-induced hyperactivity in BALB/c and black Swiss mice served as a model of mania. Behavior was evaluated in the open-field test in naïve mice or in mice treated with anti-ouabain antibodies. CS levels were determined by enzyme-linked immunosorbent assay (ELISA), using sensitive and specific anti-ouabain antibodies. Extracellular signal-regulated kinase (ERK) and protein kinase B (Akt) phosphorylation levels in the frontal cortex were determined by western blot analysis. RESULTS: Administration of AMPH to BALB/c and black Swiss mice resulted in a marked increase in locomotor activity, accompanied by a threefold increase in brain CSs. The lowering of brain CSs by the administration of anti-ouabain antibodies prevented the hyperactivity and the increase in brain CS levels. AMPH caused an increase in phosphorylated ERK (p-ERK) and phosphorylated Akt (p-Akt) levels in the frontal cortex, which was significantly reduced by administration of the antibodies. A synthetic 'functional antagonist' of CSs, 4-(3'$\alpha$-15'$\beta$-dihydroxy-5'$\beta$-estran-17'$\beta$-yl) furan-2-methyl alcohol, also resulted in attenuation of AMPH-induced hyperactivity. CONCLUSIONS: These results are in accordance with the notion that malfunctioning of the Na(+) , K(+) -ATPase/CS system may be involved in the manifestation of mania and identify this system as a potential new target for drug development.
This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains. B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations. This innovative downstream process highlights how B. bacteriovorus can be used as a novel, biological lytic agent for the inexpensive, industrial scale recovery of intracellular products from different Gram-negative prey cultures.
We demonstrate how the emergence of extreme events strongly depends on the correlation length of the input field distribution. Observing the behavior of optical waves in turbulent photorefractive propagation with partially incoherent excitations, we find that rogue waves are strongly enhanced for a characteristic input correlation scale. Waveform analysis identifies this scale with a characteristic peak-intensity-independent wave size, suggesting a general role played by saturation in the nonlinear response in rogue phenomena. [ABSTRACT FROM AUTHOR]Copyright of Physical Review A: Atomic, Molecular & Optical Physics is the property of American Physical Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder’s express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
The use of long-wavelength radiation for gate operations is a promising approach for trapped-ion quantum computation. We demonstrate the key principle of this approach by generating a maximally entangled two-qubit Bell-state with fidelity of 0.985.
BACKGROUND: The metabolite content of a seed and its ability to germinate are determined by genetic makeup and environmental effects during development. The interaction between genetics, environment and seed metabolism and germination was studied in 72 tomato homozygous introgression lines (IL) derived from Solanum pennelli and S. esculentum M82 cultivar. Plants were grown in the field under saline and fresh water irrigation during two consecutive seasons, and collected seeds were subjected to morphological analysis, gas chromatograph-mass spectrometry (GC-MS) metabolic profiling and germination tests.
RESULTS: Seed weight was under tight genetic regulation, but it was not related to germination vigor. Salinity significantly reduced seed number but had little influence on seed metabolites, affecting only 1% of the statistical comparisons. The metabolites negatively correlated to germination were simple sugars and most amino acids, while positive correlations were found for several organic acids and the N metabolites urea and dopamine. Germination tests identified putative loci for improved germination as compared to M82 and in response to salinity, which were also characterized by defined metabolic changes in the seed.
CONCLUSIONS: An integrative analysis of the metabolite and germination data revealed metabolite levels unambiguously associated with germination percentage and rate, mostly conserved in the different tested seed development environments. Such consistent relations suggest the potential for developing a method of germination vigor prediction by metabolic profiling, as well as add to our understanding of the importance of primary metabolic processes in germination.
Massimo Cogliati, D'Amicis, Roberta , Zani, Alberto , Montagna, Maria Teresa , Caggiano, Giuseppina , De Giglio, Osvalda , Balbino, Stella , De Donno, Antonella , Serio, Francesca , Susever, Serdar , Ergin, Cagri , Velegraki, Aristea , Ellabib, Mohamed S, Nardoni, Simona , Macci, Cristina , Oliveri, Salvatore , Trovato, Laura , Dipineto, Ludovico , Rickerts, Volker , McCormick-Smith, Ilka , Akcaglar, Sevim , Tore, Okan , Mlinaric-Missoni, Emilija , Bertout, Sebastien , Mallié, Michele , da Martins, Maria Luz , Vencà, Ana CF, Vieira, Maria L, Sampaio, Ana C, Pereira, Cheila , Criseo, Giuseppe , Romeo, Orazio , Ranque, Stéphane , Al-Yasiri, Mohammed HY, Kaya, Meltem , Cerikcioglu, Nilgun , Marchese, Anna , Vezzulli, Luigi , Ilkit, Macit , Desnos-Ollivier, Marie , Pasquale, Vincenzo , Korem, Maya , Polacheck, Itzhack , Scopa, Antonio , Meyer, Wieland , Ferreira-Paim, Kennio , Hagen, Ferry , Theelen, Bart , Boekhout, Teun , Lockhart, Shawn R, Tintelnot, Kathrin , Tortorano, Anna Maria , Dromer, Françoise , Varma, Ashok , Kwon-Chung, Kyung J, Inácio, Joäo , Alonso, Beatriz , ו Colom, Maria F. 2016. “Environmental Distribution Of Cryptococcus Neoformans And C. Gattii Around The Mediterranean Basin.”. Fems Yeast Research, 16, 4. doi:10.1093/femsyr/fow045. תקציר
In order to elucidate the distribution of Cryptococcus neoformans and C. gattii in the Mediterranean basin, an extensive environmental survey was carried out during 2012-2015. A total of 302 sites located in 12 countries were sampled, 6436 samples from 3765 trees were collected and 5% of trees were found to be colonized by cryptococcal yeasts. Cryptococcus neoformans was isolated from 177 trees and C. gattii from 13. Cryptococcus neoformans colonized 27% of Ceratonia, 10% of Olea, Platanus and Prunus trees and a lower percentage of other tree genera. The 13 C. gattii isolates were collected from five Eucalyptus, four Ceratonia, two Pinus and two Olea trees. Cryptococcus neoformans was distributed all around the Mediterranean basin, whereas C. gattii was isolated in Greece, Southern Italy and Spain, in agreement with previous findings from both clinical and environmental sources. Among C. neoformans isolates, VNI was the prevalent molecular type but VNII, VNIV and VNIII hybrid strains were also isolated. With the exception of a single VGIV isolate, all C. gattii isolates were VGI. The results confirmed the presence of both Cryptococcus species in the Mediterranean environment, and showed that both carob and olive trees represent an important niche for these yeasts.
Pharmaceutically active compounds are taken up and accumulate in crops irrigated with treated wastewater. This raises the concern of chronic human exposure to pharmaceuticals via food consumption. Thus, there is a need to develop a reliable technique to detect and quantify pharmaceuticals at environmentally relevant concentrations in human biological matrices, particularly urine. In this study, we focus on carbamazepine, an antiepileptic drug and recalcitrant compound that is taken up by crops—making it an excellent model compound for this study. This paper presents a new analytical technique enabling quantification of trace concentrations of carbamazepine and its metabolites in the urine of individuals who have been environmentally exposed. Sample preparation included extraction with acetonitrile followed by clean-up through mixed-mode ion-exchange cartridges and analysis using LC/MS/MS. This technique, which was validated for a wide range of concentrations (5–2000 ng L−1), exhibits low limits of quantification (3.0–7.2 ng L−1), acceptable recovery levels (70–120%), and low relative standard deviation (<20%). Unlike currently available methods for the analysis of water or treated wastewater that require large volumes (up to 1 L), the new method uses only 10 mL of urine. Moreover, relative to available methods for carbamazepine detection in the urine of individuals who are chronically treated with this drug, the limit of quantification values with our method are six orders of magnitude lower. The newly developed method has been successfully applied for the quantification of carbamazepine and its metabolites in the urine of healthy people exposed to this pharmaceutical through their diet. Our analytical protocol can provide the scientific community and stakeholders with real data for risk assessments and the design of policies ensuring safe use of wastewater for crop irrigation.
