פרסומים

The formation and local deposition of well-shaped Au nanostructures on a nonconducting surface are described. Specifically, the local electroless deposition of Au in aqueous solutions in the presence of various n-alkylpyridinium surfactants is driven by electrochemically generating a flux of AuCl4- at a gold tip close to a 3-mercaptopropyltrimethoxysilane modified Si oxidized wafer. Two reducing agents, NaBH4 and ascorbic acid, were used for the reduction of the gold ions. We studied the effect of the solution temperature, the potential applied to the gold tip and its distance from the surface, the reductant, and the nature of the alkylpyridinium on the structure of the gold deposit. The chloride salts of methylpyridinium, butylpyridinium, cetylpyridinium, 4-carbamoyl-1-cetylpyridinium, and 4-methyl-l-cetylpyridinium were added separately and showed remarkable effect on the shape of the structures that were formed. We find that short chain n-alkylpyridinium salts do not adsorb preferentially on the gold facets, whereas the longer chain n-alkylpyridinium ions cause the formation of well-faceted Au structures, such as cubes, hexagons, and even multipods. Moreover, comparison between local and bulk deposition revealed a significant difference in Au structures that were formed, presumably due to the different concentration profile of the AuCl4-.

In the present study we aimed to control insulin release from the reverse hexagonal (H-II) mesophase using Thermomyces lanuginosa lipase (TLL) in the environment (outer TLL) or within the H-II cylinders (inner TLL). Two insulin-loaded systems differing by the presence (or absence) of phosphatidylcholine (PC) were examined. In general, incorporation of PC into the H-II interface (without TLL) increased insulin release, as a more cooperative system was formed. Addition of TLL to the systems' environments resulted in lipolysis of the H-II structure. In the absence of PC, the lipolysis was more dominant and led to a significant increase in insulin release (50% after 8 h). However, the presence of PC stabilized the interface, hindering the lipolysis, and therefore no impact on the release profile was detected during the first 8 h. Entrapment of TLL within the H-II cylinders (with and without PC) drastically increased insulin release in both systems up to 100%. In the presence of PC insulin released faster and the structure was more stable. Consequently, the presence of lipases (inner or outer) both enhanced the destruction of the carrier, and provided sustained release of the entrapped insulin. (C) 2017 Elsevier B.V. All rights reserved.

Reducing the seed bank of invasive plants is a prerequisite for successful restoration of invaded ecosystems. The Australian legume tree Acacia saligna is one of the worst invasive plants in Mediterranean climate regions. This fire-adapted species possesses a large persistent seed bank characterized by physical dormant seeds. The present research was aimed to compare the efficacy of prescribed burning, soil solarization by solar heating of moist soil, and their use in combination on A. saligna seed bank reduction, using the buried seed method, and on seedling emergence from the natural seed bank, as a model of controlling fire-adapted plants. The data obtained show that the direct effect of prescribed burning on the buried seed dynamics was highly variable, and it only reduced seed viability from 98% to about 56%. Soil solarization, particularly in combination with prescribed burning, was much more effective than prescribed burning alone, reducing seed viability to about 29 and 4%, respectively. These results were confirmed by recording seedling emergence from the natural seed bank during two successive germination years following the treatments. Only a relatively very small number of seedlings emerged in the soil solarization treatment and none in the combined treatment. Based on the above data, it is recommended to apply prescribed burning as a pretreatment for soil solarization, or to utilize wild fires followed by soil solarization to reduce the seed bank of invasive fire-adapted plants. In situations in which fire cannot be used as a pretreatment, soil solarization alone is reasonably effective. © 2018, Springer International Publishing AG, part of Springer Nature.

During the complex dynamic interactions between rainfall and basin properties, different portions of the basin produce runoff at different moments. Capturing this spatiotemporal variability is important for flood analysis, but knowledge of this subject is limited. The presented research aims at improving the understanding of runoff-contributing areas (RCA; hillslope sections from which water flows, reaches the stream network, and consequently the basin outlet) and at examining their relationship with the magnitude of a flash flood’s peak discharge. A distributed hydrological model (GB-HYDRA) that enables computing RCA and flood discharge was developed. The model was applied to four medium-size basins (18–69 km2) in a Mediterranean climate and 59 flash flood events were analyzed. The correlation between basin input flux (basin area multiplied by the basin maximal rain intensity averaged over the time of concentration) and output flux (observed peak discharge) was poor (R2= 0.16). However, using a newly developed index, termed IRCA, to calculate the input flux accounting only for the RCA extent and rainfall intensity over it, resulted in a substantially higher correlation (R2= 0.64) across a wide range of flood magnitudes. The highest correlation was found using a 50-min time window, which is shorter than the time of concentration. Flood events were categorized according to their magnitude and the differences of several factors among the groups were examined. Pre-storm soil moisture content was found to be similar for all event magnitudes; however, pre-peak soil moisture content was substantially different between moderate and large–extreme events. Other important properties that differed between magnitudes were: RCA extent and its averaged rain intensity and ratio of convective rainfall. Finally, areas with land-uses characterized by low runoff potential became dominant and contributed mainly during large and extreme events. Although the RCA and its extent full potential is yet to be fulfilled, it is proposed as a significant tool for understanding processes of flash flood generation at the basin scale in future research.

MMP-14 and MMP-9 are two well-established cancer targets for which no specific clinically relevant inhibitor is available. Using a powerful combination of computational design and yeast surface display technology, we engineered such an inhibitor starting from a nonspecific MMP inhibitor, N-TIMP2. The engineered purified N-TIMP2 variants showed enhanced specificity toward MMP-14 and MMP-9 relative to a panel of off-target MMPs. MMP-specific N-TIMP2 sequence signatures were obtained that could be understood from the structural perspective of MMP/N-TIMP2 interactions. Our MMP-9 inhibitor exhibited 1000-fold preference for MMP-9 vs. MMP-14, which is likely to translate into significant differences under physiological conditions. Our results provide new insights regarding evolution of promiscuous proteins and optimization strategies for design of inhibitors with single-target specificities.

The global mental health ramifications of political violence and intimate partner violence (IPV) are well established. There also exists a growing body of evidence about the increased risks for IPV within situations of political violence. Yet, except for a few studies, there is little literature that simultaneously examines how political violence and IPV might result in unique risks for particular types of mental health sequela. Delineating possible divergent patterns between specific mental health conditions resulting from political violence and IPV takes on an increased urgency given that, although they are related, the two most commonly reported outcomes of these two types of violence—post-traumatic stress disorder (PTSD) and depression—not only require different types of treatment, but may in fact be generated or maintained by disparate paths. Using survey data from adult women in Palestine (n = 122), this study explores the relationships between IPV and political violence (both lifetime and past-month exposure) and tests their independent relationships to PTSD and depressive symptomology. After controlling for the other form of violence exposure, political violence was correlated with PTSD and not with depressive symptomology, while IPV was correlated with depressive symptomology and not with PTSD. Findings demonstrate that distinct forms of violence exposure might indeed be associated with specific mental health outcomes. Results illustrate the need to assess for both political violence and IPV when researching and designing interventions related to violence.

We present a novel gas phase detection prototype based on assembling core-shell nanospheres made of a silver core and coated with a molecularly imprinted polymer (MIP) adsorbed onto an interdigitated array (IDA) electrode chemiresistor (CR). The core-shell nanospheres, AgNP@MIPs, were imprinted with linalool, a volatile terpene alcohol, as a model system. The thickness of the MIP layer was tuned to a few nanometers to enable the facile ingress and egress of the linalool, as well as to enhance the electrical transduction through the Ag core. The AgNP@MIPs were spread onto the IDA-CR modified with various positively charged polymers, by drop casting and dip-coating. The AgNP@MIPs were characterized by various techniques such as extra high-resolution scanning and tunnelling electron microscopy and X-ray diffraction. The MIP recognition event was transduced into a measurable increase in the resistance. The response to linalool exposure and removal was fast and the device was fully recovered and could be reused. Finally, the difference in the resistance change between imprinted and non-imprinted nanospheres was substantial.

Color vision is based on the ability of different opsin proteins to tune the absorption band of their chromophore, the retinal protonated Schiff base (RPSB). Two main mechanisms proposed for this tunability are geometric and electrostatic. Here we probe the latter effect experimentally and by a quantum chemical calculation of the absorption by an isolated complex containing the retinal chromophore and molecules with a strong dipole moment. Betaine complexation causes an anomalously large blue shift. The shift provides direct evidence that the strong charge-transfer character of the electronic transition is the cause of the opsin shift, and shows that the electric field of the counterion is responsible for the color tuning, which allows absorption of light in the blue region of the visible spectrum by opsin proteins.