Orographic impact on extreme subdaily precipitation is critical for risk management but remains insufficiently understood due to complicated atmosphere-orography interactions and large uncertainties. We investigate the problem adopting a framework able to reduce uncertainties and isolate the systematic interaction of Mediterranean cyclones with a regular orographic barrier. The average decrease with elevation reported for hourly extremes is found enhanced at subhourly durations. Tail heaviness of 10-min intensities is negligibly affected by orography, suggesting self-similarity of the distributions at the convective scale. Orography decreases the tail heaviness at longer durations, with a maximum impact around hourly scales. These observations are explained by an orographically induced redistribution of precipitation toward stratiform-like processes, and by the succession of convective cores in multihour extremes. Our results imply a breaking of scale-invariance at subhourly durations, with important implications for natural hazards management in mountainous areas.
Abstract Orographic impact on extreme subdaily precipitation is critical for risk management but remains insufficiently understood due to complicated atmosphere-orography interactions and large uncertainties. We investigate the problem adopting a framework able to reduce uncertainties and isolate the systematic interaction of Mediterranean cyclones with a regular orographic barrier. The average decrease with elevation reported for hourly extremes is found enhanced at subhourly durations. Tail heaviness of 10-min intensities is negligibly affected by orography, suggesting self-similarity of the distributions at the convective scale. Orography decreases the tail heaviness at longer durations, with a maximum impact around hourly scales. These observations are explained by an orographically induced redistribution of precipitation toward stratiform-like processes, and by the succession of convective cores in multihour extremes. Our results imply a breaking of scale-invariance at subhourly durations, with important implications for natural hazards management in mountainous areas.
Protein aggregation is involved in a variety of diseases, including neurodegenerative diseases and cancer. The cellular environment is crowded by a plethora of cosolutes comprising small molecules and biomacromolecules at high concentrations, which may influence the aggregation of proteins in vivo. To account for the effect of cosolutes on cancer-related protein aggregation, we studied their effect on the aggregation of the cancer-related L106R mutant of the Axin protein. Axin is a key player in the Wnt signaling pathway, and the L106R mutation in its RGS domain results in a native molten globule that tends to form native-like aggregates. This results in uncontrolled activation of the Wnt signaling pathway, leading to cancer. We monitored the aggregation process of Axin RGS L106Rin vitro in the presence of a wide ensemble of cosolutes including polyols, amino acids, betaine and polyethylene glycol (PEG) crowders. Except myo-inositol, all polyols decreased RGS L106R aggregation, with carbohydrates exerting the strongest inhibition. Conversely, betaine and PEGs enhanced aggregation. These results are consistent with the reported effects of osmolytes and crowders on the stability of molten globular proteins and with both amorphous and amyloid aggregation mechanisms. We suggest a model of Axin L106R aggregation in vivo, whereby molecularly small osmolytes keep the protein as a free solublemolecule but the increased crowding of the bound state by macromolecules induces its aggregation at the nano-scale. Our study sheds light on the potential contribution of cosolutes to the onset of cancer as a protein misfolding disease, and on the relevance of aggregation in the molecular aetiology of cancer.
Silicon (Si) is widely accepted as a beneficial element for plants. Despite the substantial progress made in understanding Si transport mechanisms and modes of action in plants, several questions remain unanswered. In this review, we discuss such outstanding questions and issues commonly encountered by biologists studying the role of Si in plants in relation to Si bioavailability. In recent years, advances in our understanding of the role of Si-solubilizing bacteria and the efficacy of Si nanoparticles have been made. However, there are many unknown aspects associated with structural and functional features of Si transporters, Si loading into the xylem, and the role of specialized cells like silica cells and compounds preventing Si polymerization in plant tissues. In addition, despite several 1,000 reports showing the positive effects of Si in high as well as low Si-accumulating plant species, the exact roles of Si at the molecular level are yet to be understood. Some evidence suggests that Si regulates hormonal pathways and nutrient uptake, thereby explaining various observed benefits of Si uptake. However, how Si modulates hormonal pathways or improves nutrient uptake remains to be explained. Finally, we summarize the knowledge gaps that will provide a roadmap for further research on plant silicon biology, leading to an exploration of the benefits of Si uptake to enhance crop production.
Silicon (Si) is widely accepted as a beneficial element for plants. Despite the substantial progress made in understanding Si transport mechanisms and modes of action in plants, several questions remain unanswered. In this review, we discuss such outstanding questions and issues commonly encountered by biologists studying the role of Si in plants in relation to Si bioavailability. In recent years, advances in our understanding of the role of Si-solubilizing bacteria and the efficacy of Si-nanoparticles have been made. However, there are many unknown aspects associated with structural and functional features of Si transporters, Si loading into the xylem, and the role of specialized cells like silica cells and compounds preventing Si polymerization in plant tissues. In addition, despite several thousand reports showing the positive effects of Si in high as well as low Si-accumulating plant species, the exact roles of Si at the molecular level are yet to be understood. Some evidence suggests that Si regulates hormonal pathways and nutrient uptake, thereby explaining various observed benefits of Si uptake. However, how Si modulates hormonal pathways or improves nutrient uptake remains to be explained. Finally, we summarize the knowledge gaps that will provide a roadmap for further research on plant silicon biology, leading to an exploration of the benefits of Si uptake to enhance crop production.
The widespread application of solar-water-splitting for energy conversion depends on the progress of photoelectrodes that uphold stringent criteria from photoabsorber materials. After investigating almost all possible elemental and binary semiconductors, the search must be expanded to complex materials. Yet, high structural control of these materials will become more challenging with an increasing number of elements. Complex metal-oxides offer unique advantages as photoabsorbers. However, practical fabrication conditions when using glass-based transparent conductive-substrates with low thermal-stability impedes the use of common synthesis routes of high-quality metal-oxide thin-film photoelectrodes. Nevertheless, rapid thermal processing (RTP) enables heating at higher temperatures than the thermal stabilities of the substrates, circumventing this bottleneck. Reported here is an approach to overcome phase-purity challenges in complex metal-oxides, showing the importance of attaining a single-phase multinary compound by exploring large growth parameter spaces, achieved by employing a combinatorial approach to study CuBi2O4, a prime candidate photoabsorber. Pure CuBi2O4 photoelectrodes are synthesized after studying the relationship between the crystal-structures, synthesis conditions, RTP, and properties over a range of thicknesses. Single-phase photoelectrodes exhibit higher fill-factors, photoconversion efficiencies, longer carrier lifetimes, and increased stability than nonpure photoelectrodes. These findings show the impact of combinatorial approaches alongside radiative heating techniques toward discovering highly efficient multinary photoabsorbers.
PURPOSE: To characterise pain from medication-related osteonecrosis of the jaws (MRONJ) and the effects of antimicrobial treatment on it.
METHODS: Data from files of patients diagnosed with MRONJ according to the position paper of the American Association of Oral and Maxillofacial Surgeons (2014) and Multinational Association of Supportive Care in Cancer and American Society of Clinical Oncology (2019) were collected retrospectively, including gender, age, primary disease, bone-modifying agents (BMAs)/anti-angiogenics, administration route, involved jaw, location, and exposure size. The patients were treated according to the abovementioned position papers' recommendations, i.e. all patients who suffered from pain were staged as 2 or 3 and treated with systemic amoxicillin, or doxycycline or clindamycin in case of sensitivity, and local antiseptic and hygiene instructions.
RESULTS: Data from 77 MRONJ patients (aged 65.09 ± 11.9 years old) were analysed. Most (90.1%) received bisphosphonates for cancer (79%) and osteoporosis (17%). A total of 67.5% experienced pain; 36.5% had moderate-to-severe pain. Female gender was significantly associated with the presence of pain (p = 0.002). Osteonecrosis lesions after dento-alveolar surgery had a higher risk of pain development than spontaneous lesions (p = 0.045). Medical and oncologic background, type of pharmacotherapy, lesion size, and location were not associated with pain levels. Worse initial pain was significantly associated with better relief following MRONJ treatment (p = 0.045). Meaningful pain reduction (≥ 50%) was significantly correlated with initial pain severity (p = 0.0128, OR = 4.75).
CONCLUSIONS: Pain from infection and inflammation often accompanies MRONJ. The presence of pain is correlated with longer BMAs pre-therapy and if surgery preceded the MRONJ. Persistency of the mild pain together with a resistance to common antimicrobial treatment, although not complete, is a feature that MRONJ pain shares with neuropathic-"like" pain, and requires further study and consideration during treatment.
Bacteria are social organisms that interact extensively within and between species while responding to external stimuli from their environments. Designing synthetic microbial communities can enable efficient and beneficial microbiome implementation in many areas. However, in order to design an efficient community, one must consider the interactions between their members. Using a reductionist approach, we examined pairwise interactions of three related Pseudomonas species in various microenvironments including plant roots and inert surfaces. Our results show that the step between monoculture and co-culture is already very complex. Monoculture root colonization patterns demonstrate that each isolate occupied a particular location on wheat roots, such as root tip, distance from the tip, or scattered along the root. However, pairwise colonization outcomes on the root did not follow the bacterial behavior in monoculture, suggesting various interaction patterns. In addition, we show that interspecies interactions on a microscale on inert surface take part in co-culture colonization and that the interactions are affected by the presence of root extracts and depend on its source. The understanding of interrelationships on the root may contribute to future attempts to manipulate and improve bacterial colonization and to intervene with root microbiomes to construct and design effective synthetic microbial consortia.
The goal of this study was to understand the relation between adolescents’ long-term exposure to political violence (EPV) and post-traumatic stress symptoms (PTSS). We examined the relations among EPV, PTSS, and self-esteem to determine whether self-esteem could buffer the effects of EPV on PTSS. A systematic cluster random sample of 2,934 Palestinian adolescent boys and girls in the West Bank area of the Palestinian Authority and in East Jerusalem participated in a study using a self-administered questionnaire. Multiple regression analysis showed that the more Palestinian youth were exposed to political violence during the last year and during previous years, the more they exhibited PTSS and its three manifestations, that is, avoidance, intrusion, and arousal. In addition, EPV showed effects on PTSS after controlling for sociodemographic variables and self-esteem. Girls and residents of the West Bank reported more PTSS than boys and residents of East Jerusalem, respectively. Higher levels of EPV correlated with lower levels of self-esteem, and children with relatively low self-esteem were likely to report more PTSS than children with high self-esteem. Hence, the correlations between participants’ EPV and some of the PTSS were found to be partially dependent on the levels of their self-esteem, indicating moderating and mediating effects for self-esteem on the relation between degree of EPV and PTSS. The limitations of the study and implications for future research are discussed.
Steve Schulze, Yaron, Ofer , Sollerman, Jesper , Leloudas, Giorgos , Gal, Amit , Wright, Angus H, Lunnan, Ragnhild , Gal-Yam, Avishay , Ofek, Eran O, Perley, Daniel A, Filippenko, Alexei V, Kasliwal, Mansi M, Kulkarni, Shrinivas R, Neill, James D, Nugent, Peter E, Quimby, Robert M, Sullivan, Mark , Strotjohann, Nora Linn, Arcavi, Iair , Ben-Ami, Sagi , Bianco, Federica , Bloom, Joshua S, De, Kishalay , Fraser, Morgan , Fremling, Christoffer U, Horesh, Assaf , Johansson, Joel , Kelly, Patrick L, Knežević, Nikola , Knežević, Sladjana , Maguire, Kate , Nyholm, Anders , Papadogiannakis, Seméli , Petrushevska, Tanja , Rubin, Adam , Yan, Lin , Yang, Yi , Adams, Scott M, Bufano, Filomena , Clubb, Kelsey I, Foley, Ryan J, Green, Yoav , Harmanen, Jussi , Ho, Anna YQ, Hook, Isobel M, Hosseinzadeh, Griffin , D. Howell, Andrew , Kong, Albert KH, Kotak, Rubina , Matheson, Thomas , McCully, Curtis , Milisavljevic, Dan , Pan, Yen-Chen , Poznanski, Dovi , Shivvers, Isaac , van Velzen, Sjoert , ו Verbeek, Kars K. 2021. “The Palomar Transient Factory Core-Collapse Supernova Host-Galaxy Sample. I. Host-Galaxy Distribution Functions And Environment Dependence Of Core-Collapse Supernovae”. \Apjs, 255, Pp. 29. doi:10.3847/1538-4365/abff5e.
The papain-like cysteine proteases (PLCPs) are the most abundant family of cysteine proteases in plants, with essential roles in biotic/abiotic stress responses, growth and senescence. Papain, bromelain and ficin are widely used in food, medicine and other industries. In this study, 31 PLCP genes (FcPCLPs) were identified in the fig (Ficus carica L.) genome by HMM search and manual screening, and assigned to one of nine subfamilies based on gene structure and conserved motifs. SAG12 and RD21 were the largest subfamilies with 10 and 7 members, respectively. The FcPCLPs ranged from 1,128 to 5,075 bp in length, containing 1–10 introns, and the coding sequence ranged from 624 to 1,518 bp, encoding 207–505 amino acids. Subcellular localization analysis indicated that 24, 2, and 5 PLCP proteins were targeted to the lysosome/vacuole, cytoplasm and extracellular matrix, respectively. Promoter (2,000 bp upstream) analysis of FcPLCPs revealed a high number of plant hormone and low temperature response elements. RNA-seq revealed differential expression of 17 FcPLCPs in the inflorescence and receptacle, and RD21 subfamily members were the major PLCPs expressed in the fruit; 16 and 5 FcPLCPs responded significantly to ethylene and light, respectively. Proteome analyses revealed 18 and 5 PLCPs in the fruit cell soluble proteome and fruit latex, respectively. Ficins were the major PLCP in fig fruit, with decreased abundance in inflorescences, but increased abundance in receptacles of commercial-ripe fruit. FcRD21B/C and FcALP1 were aligned as the genes encoding the main ficin isoforms. Our study provides valuable multi-omics information on the FcPLCP family and lays the foundation for further functional studies.
General anesthetic agents are thought to induce loss-of-consciousness (LOC) and enable pain-free surgery by acting on the endogenous brain circuitry responsible for sleep-wake cycling. In clinical use, the entire CNS is exposed to anesthetic molecules with LOC and amnesia usually attributed to synaptic suppression in the cerebral cortex and immobility and analgesia to agent action in the spinal cord and brainstem. This model of patch-wise suppression has been challenged, however, by the observation that all functional components of anesthesia can be induced by focal delivery of minute quantities of GABAergic agonists to the brainstem mesopontine tegmental anesthesia area (MPTA). We compared spectral features of the cortical electroencephalogram (EEG) in rats during systemic anesthesia and anesthesia induced by MPTA microinjection. Systemic administration of (GABAergic) pentobarbital yielded the sustained, δ-band dominant EEG signature familiar in clinical anesthesia. In contrast, anesthesia induced by MPTA microinjection (pentobarbital or muscimol) featured epochs of δ-band EEG alternating with the wake-like EEG, the pattern typical of natural non-rapid-eye-movement (NREM) and REM sleep. The rats were not sleeping, however, as they remained immobile, atonic and unresponsive to noxious pinch. Recalling the paradoxical wake-like quality the EEG during REM sleep, we refer to this state as "paradoxical anesthesia". GABAergic anesthetics appear to co-opt both cortical and spinal components of the sleep network via dedicated axonal pathways driven by MPTA neurons. Direct drug exposure of cortical and spinal neurons is not necessary, and is probably responsible for off-target side-effects of systemic administration including monotonous δ-band EEG, hypothermia and respiratory depression. SIGNIFICANCE STATEMENT: The concept that GABAergic general anesthetic agents induce loss-of-consciousness by substituting for an endogenous neurotransmitter, thereby co-opting neural circuitry responsible for sleep-wake transitions, has gained considerable traction. However, the electroencephalographic (EEG) signatures of sleep and anesthesia differ fundamentally. We show that when the anesthetic state is generated by focal delivery of GABAergics into the mesopontine tegmental anesthesia area (MPTA) the resulting EEG repeatedly transitions between delta-wave-dominant and wake-like patterns much as in REM-NREM sleep. This suggests that systemic (clinical) anesthetic delivery, which indiscriminately floods the entire cerebrum with powerful inhibitory agents, obscures the sleep-like EEG signature associated with the less adulterated form of anesthesia obtained when the drugs are applied selectively to loci where the effective neurotransmitter substitution actually occurs.
In natural settings, many stimuli impinge on our sensory organs simultaneously. Parsing these sensory stimuli into perceptual objects is a fundamental task faced by all sensory systems. Similar to other sensory modalities, increased odor backgrounds decrease the detectability of target odors by the olfactory system. The mechanisms by which background odors interfere with the detection and identification of target odors are unknown. Here we utilized the framework of the Drift Diffusion Model (DDM) to consider possible interference mechanisms in an odor detection task. We first considered pure effects of background odors on either signal or noise in the decision-making dynamics and showed that these produce different predictions about decision accuracy and speed. To test these predictions, we trained mice to detect target odors that are embedded in random background mixtures in a two-alternative choice task. In this task, the inter-trial interval was independent of behavioral reaction times to avoid motivating rapid responses. We found that increased backgrounds reduce mouse performance but paradoxically also decrease reaction times, suggesting that noise in the decision making process is increased by backgrounds. We further assessed the contributions of background effects on both noise and signal by fitting the DDM to the behavioral data. The models showed that background odors affect both the signal and the noise, but that the paradoxical relationship between trial difficulty and reaction time is caused by the added noise.
When Gérard Genette drew the distinction between “voice” and “focus” in narrative, he pointed to two kinds of deviation from the monitoring of narrative details based on focalization. One is “paralepsis,” that is, giving the reader more information than is available to the focal character; the other is “paralipsis” – giving the reader less information than the focal character possesses. This paper suggests that the content of paralipsis – what the focal character knows but the reader is not told – is often the intentions and concrete plans of the focal character. The paper discusses the ending of Charles Dickens’s A Tale of Two Cities (1959) as a paradigmatic case: the precise intentions of Sydney Carton are not disclosed to the reader; the second reading is therefore qualitatively different from the first reading; and the intentions of the author (the implied author or even the historical author) for this temporary gap invite interpretation and raise the issue of the reasons and the causes for this feature of the narrative as a communicative act.
UV-curable particle-free ceramic compositions for stereolithography-based 3D printing technologies present a promising alternative to the commonly used particle-based compositions. So far, such compositions were mainly based on solutions of pre-ceramic polymers which limit their applications to silicon-containing materials. However, the application of particle-free inks for the fabrication of other ceramic materials, in particular dense polycrystalline ones, is very little explored. We present a new and general fabrication approach based on all-solution compositions, by combining sol–gel chemistry and photopolymerization, for obtaining dense 3D ceramic structures by DLP printing. The process is demonstrated here for the fabrication of barium titanate (BaTiO3). By using chelating solvent and monomer, a stable UV-curable solution is obtained. An aging period of 8–14 days was crucial for obtaining dense ceramic objects without any secondary phases. The heat treatment was found to affect the microstructure, density and hardness of the resulting ceramics. The presented process enables obtaining objects free of carbon materials, having a density as high as 98% of the theoretical value, and a hardness of 4.3 GPa.
