פרסומים

Despite evidence implicating microglia in the etiology and pathophysiology of major depression, there is paucity of information regarding the contribution of microglia-dependent molecular pathways to antidepressant procedures. In this study, we investigated the role of microglia in a mouse model of depression (chronic unpredictable stress—CUS) and its reversal by electroconvulsive stimulation (ECS), by examining the effects of microglia depletion with the colony stimulating factor-1 antagonist PLX5622. Microglia depletion did not change basal behavioral measures or the responsiveness to CUS, but it completely abrogated the therapeutic effects of ECS on depressive-like behavior and neurogenesis impairment. Treatment with the microglia inhibitor minocycline concurrently with ECS also diminished the antidepressant and pro-neurogenesis effects of ECS. Hippocampal RNA-Seq analysis revealed that ECS significantly increased the expression of genes related to neurogenesis and dopamine signaling, while reducing the expression of several immune checkpoint genes, particularly lymphocyte-activating gene-3 (Lag3), which was the only microglial transcript significantly altered by ECS. None of these molecular changes occurred in microglia-depleted mice. Immunohistochemical analyses showed that ECS reversed the CUS-induced changes in microglial morphology and elevation in microglial LAG3 receptor expression. Consistently, either acute or chronic systemic administration of a LAG3 monoclonal antibody, which readily penetrated into the brain parenchyma and was found to serve as a direct checkpoint blocker in BV2 microglia cultures, rapidly rescued the CUS-induced microglial alterations, depressive-like symptoms, and neurogenesis impairment. These findings suggest that brain microglial LAG3 represents a promising target for novel antidepressant therapeutics.

The Haifa bay area (HBA), which includes Mount Carmel and the Zevulun valley is the third largest metropolitan area in Israel. It is also a centre of heavy industry and an important transportation hub which serve as sources of local anthropogenic pollution. Such sources are associated with adverse health effects. In order to estimate the possible exposure of the inhabitants in such heterogeneous orographic area, a detailed atmospheric transport and dispersion modelling study is required, which in turn must take into account the local micrometeorology. The aim of this study is to conduct a spatio-temporal analysis of the flow field in the HBA in order to identify the common patterns of the average wind and characterize the statistical parameters of turbulence in this area, essential for detailed pollutants dispersion modelling. This study analyses data collected during four months of summer in a network of 16 weather stations which extend across Mount Carmel and the Zevulun valley. It was found that, during the evening and night time on Mount Carmel, different flow patterns may develop on each side, separated by the watershed line. When such conditions do not develop, as well as during the daytime, the wind field, both on Mount Carmel and the Zevulun valley is approximately homogenous. The analysis of the Monin–Obukhov similarity theory functions for the velocity standard deviations show a distinct difference between Mount Carmel and the Zevulun valley, as well as between strong and weak winds. This difference can be clearly seen also in the diurnal hourly distribution of atmospheric stabilities which exhibit higher proportions of unstable conditions in the Zevulun valley during day time and higher proportion of stable stratifications at the Mount Carmel during night-time.

 This paper argues that autonomous militaries can play a balancing role during major internal political crises. However, when militaries’ autonomy is curtailed by political leaders before the crisis, militaries cannot maintain the political balance between rulers and opponents, thereby increasing the risk of armed conflict. The paper first explains the main concepts relevant to the discussion (autonomy; political crisis; balancing role), exploring their possible inter-linkages and presenting several hypotheses. Subsequently, it discusses four relevant cases from the Middle East before and during the Arab revolts of 2010–2011: Egypt in 2011 and Lebanon in 1958, which demonstrate the balancing capacities of autonomous militaries during major political crises, and Lebanon in 1975 and Syria in 2011, which reveal that non-autonomous militaries cannot play a balancing role in such circumstances. The paper concludes with several observations regarding the military’s balancing role during major internal political crises in divided and homogenous states.  

Purpose Milk consumption is associated with increased height primarily in early childhood. However, in adolescents, data are scarce with inconsistent results. Since height is a proxy for overall health and well-being, this study evaluated the association of dairy intake with height in adolescents. Methods Students in 7th-12th grades, participating in the 2015-2016 Israeli Health and Nutrition Youth Survey, a school-based cross-sectional study, completed self-administered questionnaires, including a semi-quantitative food frequency questionnaire (n = 3529, 48% males, 15.2 +/- 1.6 years). Anthropometric measurements were also performed. Dairy servings were calculated as the calcium equivalent of 1 cup of milk, and consumption was divided into four categories from very low (< 1 serving/day) to high (3 + servings/day). BMI- and Height-for-age z scores (HAZs) were calculated according to WHO growth standard; relatively short stature (RSS) was defined as HAZ < - 0.7 SD (< 25th percentile). Multivariable linear and logistic regression analyses were performed to evaluate the association of dairy intake with HAZ and prevalence of RSS, respectively. Results Median consumption of dairy products was 2 servings/day, 1.4 from unsweetened products (milk, cheese and yogurt). Controlling for age, sex, BMI-z-score and socioeconomic status, each increment of unsweetened dairy intake was associated with on average 0.04 higher HAZ (equivalent to 0.3-0.4 cm, p < 0.05), and with reduced risk for RSS: OR 0.90, 95%CI: 0.84, 0.97, p < 0.01. No such associations were found with sweetened dairy products. Conclusion Consumption of unsweetened dairy products (3-4 servings/day) appears to contribute to achieving growth potential in adolescents. Intervention studies are necessary to determine the causal relationship between dairy intake and linear growth.

המטרה: לבחון ולהשוות את תפיסותיהם של עובדים סוציאלים והורים משכונות מוחלשות בנוגע למשמעות המושג "מצבי סיכון".   הנבדקים: 35 הורים לילדים במצבי סיכון משכונה מוחלשת ו־15 עובדים סוציאליים העובדים במחלקת הרווחה בשכונה. שיטת המחקר: הנבדקים השתתפו במחקר איכותני, שבמסגרתו נערכו ראיונות אישיים.   מן הממצאים: ניתוח הראיונות העלה שלוש תמות מרכזיות. התמה הראשונה עסקה במעורבות של העובדים הסוציאליים בשכונה. ניתוח תמה זו הראה כי בעוד שהעובדים הסוציאליים ראו במיקומה של מחלקת שירותי הרווחה בתוך השכונה כדבר מבורך, וכהזדמנות לבסס קשרים קרובים עם האוכלוסייה המקומית, נמצאו הורים שראו בקרבה זו חדירה מוגזמת למרחב שלהם. התמה השנייה התמקדה בפערי תפיסת העזרה: בעוד שההורים שהשתתפו במחקר חשו כי הם זקוקים יותר לסיוע חומרני, העובדים הסוציאליים העדיפו להציע סיוע נפשי־טיפולי. התמה השלישית והאחרונה נקשרה לתיוג המלווה את המושג "ילדים במצבי סיכון". במסגרת תמה זו נמצאו תפיסות מגוונות, הן בקרב העובדים הסוציאלים הן בקרב ההורים בנוגע למידה שבה עצם המגורים בשכונה מוחלשת וקשת יום גורם מציב בהכרח את המשפחה בסיכון.   ממצאי המחקר מצביעים על החשיבות הטמונה בהטמעת תפיסות מודעות עוני ונוגדות דיכוי בקרב עובדים סוציאליים, ובעיקר בקרב אלו שעובדים עם ילדים במצבי סיכון ומשפחותיהם.   הסבר מושג: עבודה סוציאלית מודעת־עוני - פרדיגמה בשדה המקצועי של העבודה הסוציאלית, אשר גובשה ופותחה באופן ייעודי לצורך מתן שירותים חברתיים לאנשים החיים במצב של עוני. פרדיגמה זו רואה בעוני הפרה של זכויות אדם, ומציבה במרכז את מאבקם של אנשים בעוני.  

ABSTRACTWhile scholarly attention has been devoted to social media?s potential mobilizing function, they may also contribute to demobilization discourses: social communication actively promoting nonvoting. This paper examines discourses around mobilization vs. demobilization in the context of the municipal elections in Jerusalem. As the sweeping majority of East Jerusalem Palestinians have continuously been boycotting Jerusalem?s municipal elections, this is a potent case through which to examine how demobilization functions in action, through social media conversations. Using a mixed-methods analysis of Twitter contents as structured by different languages, our findings show how mobilization and demobilization discourses can co-occur during the same election event. Users of different languages ? reflecting different social and political identities ? interpret the elections in contrasting ways, with tangible implications for (in)equality in political participation. The study thus contributes theoretically to several domains of political communication, including election studies, local politics, and language fragmentation in online political discourse.

As a confined thin sheet crumples, it spontaneously segments into flat facets delimited by a network of ridges. Despite the apparent disorder of this process, statistical properties of crumpled sheets exhibit striking reproducibility. Experiments have shown that the total crease length accrues logarithmically when repeatedly compacting and unfolding a sheet of paper. Here, we offer insight to this unexpected result by exploring the correspondence between crumpling and fragmentation processes. We identify a physical model for the evolution of facet area and ridge length distributions of crumpled sheets, and propose a mechanism for re-fragmentation driven by geometric frustration. This mechanism establishes a feedback loop in which the facet size distribution informs the subsequent rate of fragmentation under repeated confinement, thereby producing a new size distribution. We then demonstrate the capacity of this model to reproduce the characteristic logarithmic scaling of total crease length, thereby supplying a missing physical basis for the observed phenomenon.

Induced polarization (IP) is increasingly applied for hydrological, environmental and agricultural purposes. Interpretation of IP data is based on understanding the relationship between the IP signature and the porous media property of interest. Mechanistic models on the IP phenomenon rely on the Poisson-Nernst-Plank equations, where diffusion and electromigration fluxes are the driving forces of charge transport and are directly related to IP. However, to our knowledge, the impact of advection flux on IP was not investigated experimentally and was not considered in any IP model. In this work, we measured the spectral IP (SIP) signature of porous media under varying flow conditions, in addition to developing and solving a model for SIP signature of porous media, which takes flow into consideration. The experimental and the model results demonstrate that as bulk velocity increases, polarization and relaxation time decrease. Using a numerical model, we established that fluid flow near the particle deforms the electrical double layer (EDL) structure, accounting for the observed reduction in polarization. We found a qualitative agreement between the model and the measurements. Still, the model overestimates the impact of flow rate on SIP signature, which we explain in terms of the flow boundary conditions. Overall, our results demonstrate the sensitivity of the SIP signature to fluid flow, highlighting the need to consider fluid velocity in the interpretation of the SIP signature of porous media, and opening an exciting new direction for noninvasive measurements of fluid flow at the EDL scale.

Monitoring the growth, architecture, and function of plant roots is of great interest. One promising noninvasive geoelectrical monitoring approach is spectral induced polarization (SIP). However, the roots' SIP signature is underexplored, not well understood, and a mechanistic model has not been proposed. Here, we developed a mechanistic model for SIP's response of roots, which is based on the Poisson-Nernst-Planck equation. The modeling results suggest that the magnitude of root polarization is linearly related to the root's external surface area and that the polarization length scale is the root's diameter. We suggest that injecting a current to the plant's stem results in higher polarization associated with the root-cells' total surface area. In this case, the polarization length scale is the cell diameter. Overall, we quantified the link between the root's dimensions and their electrical signature, which may inspire SIP application for root phenotyping.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a severe global pandemic. Mice models are essential to investigate infection pathology, antiviral drugs, and vaccine development. However, wild-type mice lack the human angiotensin-converting enzyme 2 (hACE2) that mediates SARS-CoV-2 entry into human cells and consequently are not susceptible to SARS-CoV-2 infection. hACE2 transgenic mice could provide an efficient COVID-19 model, but are not always readily available, and practically restricted to specific strains. Therefore, there is a dearth of additional mouse models for SARS-CoV-2 infection. We applied lentiviral vectors to generate hACE2 expression in interferon receptor knock-out (IFNAR1(-/-)) mice. Lenti-hACE2 transduction supported SARS-CoV-2 replication in vivo, simulating mild acute lung disease. Gene expression analysis revealed two modes of immune responses to SARS-CoV-2 infection: one in response to the exposure of mouse lungs to SARS-CoV-2 particles in the absence of productive viral replication, and the second in response to productive SARS-CoV-2 infection. Our results infer that immune response to immunogenic elements on incoming virus or in productively infected cells stimulate diverse immune effectors, even in absence of type I IFN signaling. Our findings should contribute to a better understanding of the immune response triggered by SARS-CoV-2 and to further elucidate COVID-19.

Drought intensity as experienced by plants depends upon soil moisture status and atmospheric variables such as temperature, radiation, and air vapour pressure deficit (VPD). Although the role of shoot architecture with these edaphic and atmospheric factors is well-characterized, the extent to which shoot and root dynamic interactions as a continuum are controlled by genotypic variation is less known. Here, we targeted these interactions using a wild emmer introgression line (IL20) with a distinct drought-induced shift in the shoot-to-root ratio and its drought-sensitive recurrent parent Svevo. Using a gravimetric platform, we show that IL20 maintained higher root water influx and gas exchange under drought stress, which supported a greater growth. Interestingly, the advantage of IL20 in root water influx and transpiration was expressed earlier during the daily diurnal cycle under lower VPD and therefore supported higher transpiration efficiency. Application of structural equation model indicates that under drought, VPD and radiation are antagonistic to transpiration rate, whereas the root water influx operates as feedback for the higher atmospheric responsiveness of leaves. Collectively, our results suggest that a drought-induced shift in root-to-shoot ratio can improve plant water uptake potential in a short preferable time window determined by both water and atmospheric parameters.

Drought intensity as experienced by plants depends upon soil moisture status and atmospheric variables such as temperature, radiation, and air vapour pressure deficit. Although the role of shoot architecture with these edaphic and atmospheric factors is well characterized, the extent to which shoot and root dynamic interactions as a continuum are controlled by genotypic variation is less well known. Here, we targeted these interactions using a wild emmer wheat introgression line (IL20) with a distinct drought-induced shift in the shoot-to-root ratio and its drought-sensitive recurrent parent Svevo. Using a gravimetric platform, we show that IL20 maintained higher root water influx and gas exchange under drought stress, which supported a greater growth. Interestingly, the advantage of IL20 in root water influx and transpiration was expressed earlier during the daily diurnal cycle under lower vapour pressure deficit and therefore supported higher transpiration efficiency. Application of a structural equation model indicates that under drought, vapour pressure deficit and radiation are antagonistic to transpiration rate, whereas the root water influx operates as a feedback for the higher atmospheric responsiveness of leaves. Collectively, our results suggest that a drought-induced shift in root-to-shoot ratio can improve plant water uptake potential in a short preferable time window during early morning when vapour pressure deficit is low and the light intensity is not a limiting factor for assimilation.