פרסומים

A qualitative study examined children's subjective perception of their mother's situation during their stay in shelters for battered women (SBW). Thirty-two children, aged 7–12 years, who were staying with their mothers in SBWs participated in this study. Thematic analysis revealed the following two core themes: children's perceptions and insights, and feelings that were related to their perceptions. The findings are discussed in light of the concepts of exposure to IPV as a lived trauma, and re-exposure to violence in new contexts, and the role of the relationship with the abused mother in shaping the child's well-being.

In nature, frost can form at a few degrees below 0 °C. However, this process requires the assembly of tens of thousands of ice-like water molecules that align together to initiate freezing at these relatively high temperatures. Water ordering on this scale is mediated by the ice nucleation proteins (INPs) of common environmental bacteria like Pseudomonas syringae and Pseudomonas borealis. However, individually, these 100 kDa proteins are too small to organize enough water molecules for frost formation, and it is not known how giant, megadalton-sized multimers, which are crucial for ice nucleation at high sub-zero temperatures, form. The ability of multimers to self-assemble was suggested when the transfer of an INP gene into Escherichia coli led to efficient ice nucleation. Here, we demonstrate that a positively charged subdomain at the C-terminal end of the central β-solenoid of the INP is crucial for multimerization. Truncation, relocation, or change of the charge of this subdomain caused a catastrophic loss of ice nucleation ability. Cryo-electron tomography of the recombinant E. coli showed that the INP multimers form fibres that are ~5 nm across and up to 200 nm long. A model of these fibres as an overlapping series of antiparallel dimers can account for all their known properties and suggests a route to making cell-free ice nucleators for biotechnological applications.

Thermal intolerance may limit activity in hostile environments. After heat illness, two physiologically distinct phenotypes evolve: heat tolerant (HT) and heat intolerant (HI). The recognition that heat illness alters gene expression justified revisiting the established physiological concept of HI. We used a DNA microarray to examine the global transcriptional response in peripheral blood mononuclear cells (PMBCs) from HI and HT phenotypes, categorized 2-mo postheat injury using a functional physiological heat-tolerance test (HTT, 40°C)-Recovery (R, 24°C) protocol. The impact of recurrent heat stress was studied in vitro using peripheral blood mononuclear cells (PBMCs) from controls (participants with no history of heat injury), HI, and HT (categorized by functional HTT) with a customized NanoString array. There were significant differences under basal conditions between the HI and HT. HI were more immunological alerted. Almost no shared genes were found between end-HTT and recovery phases, suggesting vast cellular plasticity. In HI, mitochondrial function was dysregulated, canonical pathways associated with exercise endurance-NRF2 and insulin were downregulated, whereas AMPK and peroxisome proliferator-activated receptor (PPAR) were upregulated. HT exhibited reciprocal responses, suggesting that energy dysregulation found in HI interfered with performance in the heat. The endoplasmic-reticulum stress response was also suppressed in HI. In vitro HTT (43°C) abolished differences between HI and HT PBMCs including the HSPs genes, whereas controls showed profound HSPs upregulation.

The impact of climate on topography, which is a theme in landscape evolution studies, has been demonstrated, mostly, at mountain range scales and across climate zones. However, in drylands, spatiotemporal discontinuities of rainfall and the crucial role of extreme rainstorms raise questions and challenges in identifying climate properties that govern surface processes. Here, we combine methods to examine hyperarid escarpment sensitivity to storm-scale forcing. Using a high-resolution DEM and field measurements, we analyzed the topography of a 40-km-long escarpment in the Negev desert (Israel). We also used rainfall intensity data from a convection-permitting numerical weather model for storm-scale statistical analysis. We conducted hydrological simulations of synthetic rainstorms, revealing the frequency of sediment mobilization along the sub-cliff slopes. Results show that cliff gradients along the hyperarid escarpment increase systematically from the wetter (90 mm yr−1) southwestern to the drier (45 mm yr−1) northeastern sides. Also, sub-cliff slopes at the southwestern study site are longer and associated with milder gradients and coarser sediments. Storm-scale statistical analysis reveals a trend of increasing extreme (>10 years return-period) intensities toward the northeast site, opposite to the trend in mean annual rainfall. Hydrological simulations based on these statistics indicate a higher frequency of sediment mobilization in the northeast, which can explain the pronounced topographic differences between the sites. The variations in landscape and rainstorm properties across a relatively short distance highlight the sensitivity of arid landforms to extreme events.

We propose a model that simulates climate change impacts on crop production and food prices under partial equilibrium. Our model incorporates a system of Laspeyres price and quantity indices that link structurally estimated community-level produce supply functions to market level demand functions. The supply estimation accounts for corner solutions associated with disaggregate land use observations and is constrained to reproduce aggregate supply data. We use the model to assess climate change impacts in Israel, which protects local agriculture by import tariffs and quotas. The simulation results vary greatly when we allow prices to change as a response to supply changes, highlighting the importance of endogenizing prices in climate change simulations. The results imply that climate changes projected for Israel are expected to be beneficial to farmers, particularly due to the positive impact of the forecasted large temperature rise on field crop production. Fruit outputs are projected to decline, and reduce consumer surplus, but to a lower extent than the increase in total agricultural profits. Nearly 20% of the profit rise is attributed to farmers’ adaptation through land reallocation. Adaptation to the projected reduction in precipitation by increasing irrigation is found to be warranted from the farmers’ perspective; however, it is not beneficial to society as a whole. Abolishing import tariffs effectively transfers surpluses from producers to consumers, but the impact of this policy on social welfare is relatively modest.We propose a model that simulates climate change impacts on crop production and food prices under partial equilibrium. Our model incorporates a system of Laspeyres price and quantity indices that link structurally estimated community-level produce supply functions to market level demand functions. The supply estimation accounts for corner solutions associated with disaggregate land use observations and is constrained to reproduce aggregate supply data. We use the model to assess climate change impacts in Israel, which protects local agriculture by import tariffs and quotas. The simulation results vary greatly when we allow prices to change as a response to supply changes, highlighting the importance of endogenizing prices in climate change simulations. The results imply that climate changes projected for Israel are expected to be beneficial to farmers, particularly due to the positive impact of the forecasted large temperature rise on field crop production. Fruit outputs are projected to decline, and reduce consumer surplus, but to a lower extent than the increase in total agricultural profits. Nearly 20% of the profit rise is attributed to farmers’ adaptation through land reallocation. Adaptation to the projected reduction in precipitation by increasing irrigation is found to be warranted from the farmers’ perspective; however, it is not beneficial to society as a whole. Abolishing import tariffs effectively transfers surpluses from producers to consumers, but the impact of this policy on social welfare is relatively modest.

The biosensing of bacterial pathogens is of a high priority. Electrochemical biosensors are an important future tool for rapid bacteria detection. A monolayer of bacterial-binding peptides can serve as a recognition layer in such detection devices. Here, we explore the potential of random peptide mixtures (RPMs) composed of phenylalanine and lysine in random sequences and of controlled length, to form a monolayer that can be utilized for sensing. RPMs were found to assemble in a thin and diluted layer that attracts various bacteria. Faradaic electrochemical impedance spectroscopy was used with modified gold electrodes to measure the charge-transfer resistance (RCT) caused due to the binding of bacteria to RPMs. Pseudomonas aeruginosa was found to cause the most prominent increase in RCT compared to other model bacteria. We show that the combination of highly accessible antimicrobial RPMs and electrochemical analysis can be used to generate a new promising line of bacterial biosensors.