פרסומים

{BACKGROUND AND OBJECTIVE: An inconsistent association between exposure to SSRIs and SNRIs and the risk for ASD and ADHD in the Offspring was observed in observational studies. Some suggest that the reported association might be due to unmeasured confounding. We aimed to study this association and to look for sources of bias by performing a systematic review and meta-analysis. METHODS: Medline, Embase, and the Cochrane Library were searched up to June 2019 for studies reporting on ASD and ADHD in the Offspring following exposure during pregnancy. We followed the PRISMA 2009 guidelines for data selection and extraction. Outcomes were pooled using random- effects models and odds ratios (OR), and 95% confidence intervals (CI) were calculated for each outcome using the adjusted point estimate of each study. RESULTS: Eighteen studies were included in the meta-analysis. We found an association between SSRIs/ SNRIs prenatal use and the risk for ASD and ADHD (OR=1.42, 95% CI: 1.23-1.65, I(2)=58%; OR=1.26, 95% CI: 1.07-1.49

One of the main challenges in cancer surgery is to ensure the complete excision of the tumour while sparing as much healthy tissue as possible. Histopathology, the gold-standard technique used to assess the surgical margins on the excised tissue, is often impractical for intra-operative use because of the time-consuming tissue cryo-sectioning and staining, and availability of histopathologists to assess stained tissue sections. Raman micro-spectroscopy is a powerful technique that can detect microscopic residual tumours on ex vivo tissue samples with accuracy, based entirely on intrinsic chemical differences. However, raster-scanning Raman micro-spectroscopy is a slow imaging technique that typically requires long data acquisition times wich are impractical for intra-operative use. Selective-sampling Raman imaging overcomes these limitations by using information regarding the spatial properties of the tissue to reduce the number of Raman spectra. This paper reviews the latest advances in selective-sampling Raman techniques and applications, mainly based on multimodal optical imaging. We also highlight the latest results of clinical integration of a prototype device for non-melanoma skin cancer. These promising results indicate the potential impact of Raman spectroscopy for providing fast and objective assessment of surgical margins, helping surgeons ensure the complete removal of tumour cells while sparing as much healthy tissue as possible.

Background. Clinical diagnosis of coronavirus disease 2019 (COVID-19) is essential to the detection and prevention of COVID-19. Sudden onset of loss of taste and smell is a hallmark of COVID-19, and optimal ways for including these symptoms in the screening of patients and distinguishing COVID-19 from other acute viral diseases should be established. Methods. We performed a case-control study of patients who were polymerase chain reaction-tested for COVID-19 (112 positive and 112 negative participants), recruited during the first wave (March 2020-May 2020) of the COVID-19 pandemic in Israel. Patients reported their symptoms and medical history by phone and rated their olfactory and gustatory abilities before and during their illness on a 1-10 scale. Results. Changes in smell and taste occurred in 68% (95% CI, 60%-76%) and 72% (95% CI, 64%-80%) of positive patients, with odds ratios of 24 (range, 11-53) and 12 (range, 6-23), respectively. The ability to smell was decreased by 0.5 +/- 1.5 in negatives and by 4.5 +/- 3.6 in positives. A penalized logistic regression classifier based on 5 symptoms had 66% sensitivity, 97% specificity, and an area under the receiver operating characteristics curve (AUC) of 0.83 on a holdout set. A classifier based on degree of smell change was almost as good, with 66% sensitivity, 97% specificity, and 0.81 AUC. The predictive positive value of this classifier was 0.68, and the negative predictive value was 0.97. Conclusions. Self-reported quantitative olfactory changes, either alone or combined with other symptoms, provide a specific tool for clinical diagnosis of COVID-19. A simple calculator for prioritizing COVID-19 laboratory testing is presented here. [GRAPHICS] .

Environmental stresses are among the major factors that limit crop productivity and plant growth. Various nondestructive approaches for monitoring plant stress states have been developed. However, early sensing of the initial biochemical events during stress responses remains a significant challenge. In this work, we established whole-plant redox imaging using potato (Solanum tuberosum) plants expressing a chloroplast-targeted redox-sensitive green fluorescence protein 2 (roGFP2), which reports the glutathione redox potential (E-GSH). Ratiometric imaging analysis demonstrated the probe response to redox perturbations induced by H2O2, DTT, or a GSH biosynthesis inhibitor. We mapped alterations in the chloroplast E-GSH under several stress conditions including, high-light (HL), cold, and drought. An extremely high increase in chloroplast E-GSH was observed under the combination of HL and low temperatures, conditions that specifically induce PSI photoinhibition. Intriguingly, we noted a higher reduced state in newly developed compared with mature leaves under steady-state and stress conditions, suggesting a graded stress sensitivity as part of the plant strategies for coping with stress. The presented observations suggest that whole-plant redox imaging can serve as a powerful tool for the basic understanding of plant stress responses and applied agricultural research, such as toward improving phenotyping capabilities in breeding programs and early detection of stress responses in the field.

The idea that individuals differ in their sensitivity to the environment's effects is a cornerstone of developmental science. It has been demonstrated repeatedly, for different kinds of stressors, outcomes, and sensitivity markers. However, almost no empirical work was done to examine whether environmental sensitivity is domain-general (i.e., the same individuals are sensitive to different environmental contexts) or domain-specific (i.e., different individuals are sensitive to different environmental contexts), despite its importance to understanding human development, learning, and behavior. To address this question, phenotypic sensitivity to parents and to peers were compared in 1313 11-year-old Israeli adolescent twins. We found that, (1) our phenotypic markers indeed moderate environmental influences, with a discriminant predictive utility, (2) adolescents who are sensitive to their parents are not necessarily sensitive to their peers, and (3) sensitivity to parents and sensitivity to peers have different etiologies and show a negative genetic correlation, indicating that adolescents carrying genetic markers for sensitivity to parents are less likely to carry genetic markers for sensitivity to peers. These findings suggest that environmental sensitivity shows domain-specific patterns, as different individuals can be sensitive to different environments. We discuss the theoretical, empirical, and practical implications of domain-specificity of environmental sensitivity.

The idea that individuals differ in their sensitivity to the environment's effects is a cornerstone of developmental science. It has been demonstrated repeatedly, for different kinds of stressors, outcomes, and sensitivity markers. However, almost no empirical work was done to examine whether environmental sensitivity is domain-general (i.e., the same individuals are sensitive to different environmental contexts) or domain-specific (i.e., different individuals are sensitive to different environmental contexts), despite its importance to understanding human development, learning, and behavior. To address this question, phenotypic sensitivity to parents and to peers were compared in 1313 11-year-old Israeli adolescent twins. We found that, (1) our phenotypic markers indeed moderate environmental influences, with a discriminant predictive utility, (2) adolescents who are sensitive to their parents are not necessarily sensitive to their peers, and (3) sensitivity to parents and sensitivity to peers have different etiologies and show a negative genetic correlation, indicating that adolescents carrying genetic markers for sensitivity to parents are less likely to carry genetic markers for sensitivity to peers. These findings suggest that environmental sensitivity shows domain-specific patterns, as different individuals can be sensitive to different environments. We discuss the theoretical, empirical, and practical implications of domain-specificity of environmental sensitivity.

Problem, research strategy, and findings

The current focus on power relationships in planning processes emphasizes socioeconomic characteristics of the general public, whose participation is often portrayed as one-time, idiosyncratic, nonprofessional, and relatively powerless. To shift attention to the understudied repeated participation in the general public, we distinguish serial participation as a distinct participation pattern by focusing on an underexplored group, referred to as natural joiners or usual suspects. Our analysis focuses on Jerusalem and draws on interviews with serial participators (N = 13) who participated in at least three different planning processes and with city planners (N = 19). Becoming a serial participator emerged as an evolutionary process, during which knowledge gained triggered transitional learning, manifested by a broader perspective on planning and a transition toward locality-oriented participation. Serial participators’ influence varies; it can extend beyond specific planning outcomes to the process itself and the discourse among city planners. Although it does not mitigate imbalanced power relations within the public, serial participation contributes to more balanced power relations between ordinary citizens and paid participants in planning.

Takeaway for practice

Serial participators can provide planners with valuable historical perspective, local knowledge, and participation recommendations while serving as intermediaries to the local community capable of mobilizing others and activating civic networks. Planners can nurture serial participation by encouraging repeated involvement of individuals engaged in additional community spheres or passionate “anecdotal” participants. Seeking influence and not recognition, serial participators may not always fully cooperate. Planners should invest in long-term relationships that allow for reconciling inevitable disagreements.

Aims: Feeding regimens alter circadian rhythms in peripheral tissues, but the mechanism is not understood. We aimed to study whether soluble factors, rather than neuronal-based communication, directly influence circadian rhythms in the liver, in response to a nutritional treatment in type 2 diabetes (T2D) patients. Methods: Cultured hepatocytes were treated with serum of insulin-treated T2D patients following either a three-meal diet (3Mdiet) or six-meal diet (6Mdiet) and the circadian expression of clock and metabolic genes was measured. Results: Serum of the 3Mdiet group led to increased amplitudes and daily mRNA levels of the positive limb of the circadian clock (Clock, Bmal1, Rora). In parallel, serum of the 3Mdiet group led to the downregulation of the negative limb of the circadian clock (Cry1 and Per1), compared to both baseline and 6Mdiet. In contrast, serum of the 6Mdiet group led to a more distorted expression pattern. The catabolic genes Sirt1 and Ampk were significantly upregulated only by serum of the 3Mdiet group. Conclusions: Our results show that serum of type 2 diabetes patients consuming the 3Mdiet contains soluble factors that reset circadian rhythms leading to an expression pattern similar to that of healthy people. This clock pattern contributes to improved glucose metabolism. (c) 2021 Elsevier B.V. All rights reserved.

There is growing evidence that symbiotic microbes can influence multiple nutrition-related behaviors of their hosts, including locomotion, feeding, and foraging. However, how the microbiome affects nutrition-related behavior is largely unknown. Here, we demonstrate clear sexual dimorphism in how the microbiome affects foraging behavior of a frugivorous fruit fly, Drosophila suzukii. Female flies deprived of their microbiome (axenic) were consistently less active in foraging on fruits than their conventional counterparts, even though they were more susceptible to starvation and starvation-induced locomotion was notably more elevated in axenic than conventional females. Such behavioral change was not observed in male flies. The lag of axenic female flies but not male flies to forage on fruits is associated with lower oviposition by axenic flies, and mirrored by reduced food seeking observed in virgin females when compared to mated, gravid females. In contrast to foraging intensity being highly dependent on the microbiome, conventional and axenic flies of both sexes showed relatively consistent and similar fruit preferences in foraging and oviposition, with raspberries being preferred among the fruits tested. Collectively, this work highlights a clear sex-specific effect of the microbiome on foraging and locomotion behaviors in flies, an important first step toward identifying specific mechanisms that may drive the modulation of insect behavior by interactions between the host, the microbiome, and food.

There is growing evidence that symbiotic microbes can influence multiple nutrition-related behaviors of their hosts, including locomotion, feeding, and foraging. However, how the microbiome affects nutrition-related behavior is largely unknown. Here, we demonstrate clear sexual dimorphism in how the microbiome affects foraging behavior of a frugivorous fruit fly, Drosophila suzukii. Female flies deprived of their microbiome (axenic) were consistently less active in foraging on fruits than their conventional counterparts, even though they were more susceptible to starvation and starvation-induced locomotion was notably more elevated in axenic than conventional females. Such behavioral change was not observed in male flies. The lag of axenic female flies but not male flies to forage on fruits is associated with lower oviposition by axenic flies, and mirrored by reduced food seeking observed in virgin females when compared to mated, gravid females. In contrast to foraging intensity being highly dependent on the microbiome, conventional and axenic flies of both sexes showed relatively consistent and similar fruit preferences in foraging and oviposition, with raspberries being preferred among the fruits tested. Collectively, this work highlights a clear sex-specific effect of the microbiome on foraging and locomotion behaviors in flies, an important first step toward identifying specific mechanisms that may drive the modulation of insect behavior by interactions between the host, the microbiome, and food.

Scattering by atmospheric turbulence is one of the main challenges in creating long free-space optical links, and specifically links of entangled photons. Classical compensation methods are hard to apply to entangled photons, due to inherently low signal to noise ratios and the fragility of entanglement. We have recently shown that we can use a bright laser beam that pumps spontaneous parametric down conversion to control the spatial correlations between entangled photons for compensating their scattering. In this work, we apply the pump-shaping technique to compensate for the scrambling of correlations between entangled photons that scatter by emulated atmospheric turbulence. We use a spatial light modulator and Kolmogorov&\#x2019;s turbulence model to emulate atmospheric turbulence in the lab, and enhance the entangled photons&\#x2019; signal by a factor of fifteen using pump optimization. We show this for both a static and dynamic emulated atmosphere, and also demonstrate the compensation of the scattering of a higher-order mode. Our results can open the door towards realizing free-space quantum links with entangled photons, used in applications such as quantum key distribution.