TY - JOUR T1 - Combinatorial and Computational Approaches to Identify Interactions of Macrophage Colony-stimulating Factor (M-CSF) and Its Receptor c-FMS JF - J Biol Chem Y1 - 2015 A1 - Rosenfeld, L. A1 - Shirian, J. A1 - Zur, Y. A1 - Levaot, N. A1 - Shifman, J. M. A1 - Papo, N. KW - Combinatorial Chemistry Techniques KW - computer modeling KW - directed evolution KW - epitope mapping KW - Flow Cytometry KW - Humans KW - ligand-binding protein KW - Macrophage Colony-Stimulating Factor/chemistry/*metabolism KW - Protein Binding KW - Protein Conformation KW - receptor tyrosine kinase KW - Receptor, Macrophage Colony-Stimulating Factor/*metabolism AB - The molecular interactions between macrophage colony-stimulating factor (M-CSF) and the tyrosine kinase receptor c-FMS play a key role in the immune response, bone metabolism, and the development of some cancers. Because no x-ray structure is available for the human M-CSF . c-FMS complex, the binding epitope for this complex is largely unknown. Our goal was to identify the residues that are essential for binding of the human M-CSF to c-FMS. For this purpose, we used a yeast surface display (YSD) approach. We expressed a combinatorial library of monomeric M-CSF (M-CSFM) single mutants and screened this library to isolate variants with reduced affinity for c-FMS using FACS. Sequencing yielded a number of single M-CSFM variants with mutations both in the direct binding interface and distant from the binding site. In addition, we used computational modeling to map the identified mutations onto the M-CSFM structure and to classify the mutations into three groups as follows: those that significantly decrease protein stability; those that destroy favorable intermolecular interactions; and those that decrease affinity through allosteric effects. To validate the YSD and computational data, M-CSFM and three variants were produced as soluble proteins; their affinity and structure were analyzed; and very good correlations with both YSD data and computational predictions were obtained. By identifying the M-CSFM residues critical for M-CSF . c-FMS interactions, we have laid down the basis for a deeper understanding of the M-CSF . c-FMS signaling mechanism and for the development of target-specific therapeutic agents with the ability to sterically occlude the M-CSF.c-FMS binding interface. VL - 290 SN - 1083-351X (Electronic)0021-9258 (Linking) N1 - Rosenfeld, LiorShirian, JasonZur, YuvalLevaot, NoamShifman, Julia MPapo, NivengResearch Support, Non-U.S. Gov't2015/09/12 06:00J Biol Chem. 2015 Oct 23;290(43):26180-93. doi: 10.1074/jbc.M115.671271. Epub 2015 Sep 10. U2 - PMC4646268 ER -