פרסומים

The field of printed electronics is continually trying to reduce the dimensions of the elec. components. Here, a method of printing metallic lines with widths as small as 15 nm and up to a few micrometers using fountain pen nanolithog. (FPN) is shown. The FPN technique is based on a bent nanopipette with at. force feedback that acts similar to a nanopen. The geometry of the nanopen allows for rapid placement accuracy of the printing tip, on any desired location, with the highest of optical sub-micrometer resoln. Using this nanopen, investigations of various inks are undertaken together with instrumental and script-tool development that allows accurate printing of multiple layers. This has led to the printing of conductive lines using inks composed of silver nanoparticles and salt solns. of silver and copper. In addn., it is shown that the method can be applied to substrates of various materials with minimal effect on the dimension of the line. The line widths are varied by using nanopens with different orifices or by tailoring the wetting properties of the ink on the substrate. Metallic interconnections of conducting lines are reported. [on SciFinder(R)]

Cellulose, a major constituent of our natural environment and a structured biodegradable biopolymer, has been shown to exhibit shear piezoelectricity with potential applications in energy harvesters, biomedical sensors, electro-active displays and actuators. In this regard, a high-aspect ratio nanofiber geometry is particularly attractive as flexing or bending will likely produce a larger piezoelectric response as compared to axial deformation in this material. Here we report self-assembled cellulose nanofibers (SA-CNFs) fabricated using a template-wetting process, whereby parent cellulose nanocrystals (CNCs) introduced into a nanoporous template assemble to form rod-like cellulose clusters, which then assemble into SA-CNFs. Annealed SA-CNFs were found to exhibit an anisotropic shear piezoelectric response as directly measured using non-destructive piezo-response force microscopy (ND-PFM). We interpret these results in light of the distinct hierarchical structure in our template-grown SA-CNFs as revealed by scanning electron microscopy (SEM) and high resolution transmission electron microscopy (TEM).

The Israel - National Ecosystem Assessment (I-NEA) project aims to present a comprehensive picture of the state and trends of Israel’s ecosystem services across all ecosystems, by integrating existing data and information collected from a wide range of sources. Although there is a lack of information about the spatial distribution of ecosystem services’ provisioning in Israel, their mapping constitutes an important part of the assessment. In this paper, we present a national-scale mapping of three ecosystem services, each of them implemented using different methods: 1) Genetic resources service, mapped using spatial observations of the Crop Wild Relatives species; 2) potential of pollination service, which is provided by wild bees, mapped using an expert-based habitat model related to land use and land cover; and 3) cultural service of recreation, mapped by analysing the distribution of geotagged digital photographs uploaded to social media resources. The derived maps visualise, for the first time in Israel, the spatially distributed values of the three ecosystem services. Supply hotspots with high values for all three services were identified, as well as spatial differences amongst the ecosystem services. These nationalscale maps provide overlooked insights and can be very useful for strategic discussions of stakeholders and decision-makers but should be regarded with caution given existing knowledge gaps and possible inaccuracies due to data scarcity and low resolution. © Lotan A et al.

The nature-nurture debate regarding the origin of mental lines is fundamental for cognitive neuroscience. We examined natural-nurture effects on the mental time line, applying three different challenges to the directionality of time representation. We tested (1) patients with left-neglect and healthy participants, who are (2) left-to-right or right-to-left readers/writers, using (3) a lateralized left-right button press or a vocal mode in response to a mental time task, which asks participants to judge whether events have already happened in the past or are still to happen in the future. Using lateralized responses, a spatial-temporal association of response code (STEARC) effect was found, in concordance with the cultural effects. With vocal responses (no lateralization), past and future events showed similar results in both cultures. In patients with neglect, who have a deficit of spatial attention in processing the left side of space, future events were processed more slowly and less accurately than past events in both cultures. Our results indicate the existence of a “natural” disposition to map past and future events along a horizontal mental time line, which is affected by the different ways in which spatial representation of time is introduced.

The article draws on the first findings of the INFOCORE project to better understand the ways in which different types of media matter to the emergence, escalation or, conversely, the pacification and prevention of violence. The authors make the case for combining an interactionist approach of media influence, which is centred on the effects of evidential claims, frames and agendas made by various actors over time, with greater sensitivity for the factors that make conflict cases so different. They argue that the specific role played by the media depends, chiefly: (a) on the ways in which it transforms conflict actors’ claims, interpretations and prescriptions into media content; and (b) their ability to amplify these contents and endow them with reach, visibility and consonance. They found significant variation in media roles across six conflict cases and suggest that they are best explained four interlocking conditioning factors: (i) the degree to which the media landscape is diverse and free, or conversely, controlled and instrumentalized by conflict parties; (ii) societal attitudes to and uses of different media by audiences; (iii) different degrees of conflict intensity and dynamics between the conflict parties; (iv) the degree and nature of the involvement of regional and international actors. The article maintains that de-escalatory media influence will be most effective over the longer term, in settings of low intensity conflict and when tailored carefully to local conditions.

Neural stem cells (NSCs) are self-renewing multipotent cells that line the neural-tube and generate all the nervous system. Understanding NSC biology is fundamental for neurodevelopmental research and therapy. Many studies emphasized the need to culture NSCs, which are typically purified from mammalian embryonic/adult brains. These sources are somewhat limited in terms of quantity, availability and animal ethical guidelines. Therefore, new sources are needed. The chick is a powerful system for experimental embryology which contributed enormously to neurodevelopmental concepts. Its accessibility, genetic/molecular manipulations, and homology to other vertebrates, makes it valuable for developmental biology research. Recently, we identified a population of NSCs in the chick hindbrain. It resides in rhombomere-boundaries, expresses Sox2 and generates progenitors and neurons. Here, we investigated whether these cells can recapitulate hindbrain development in culture. By developing approaches to propagate and image cells, manipulate their growth-conditions and separate them into subpopulations, we demonstrate the ordered formation of multipotent and self-renewing neurospheres that maintain regional identity and display differential stem/differentiation/proliferation properties. Live imaging revealed new cellular dynamics in the culture. Collectively, these NSC cultures reproduce major aspects of hindbrain development in-vitro, proposing the chick as a model for culturing hindbrain-NSCs that can be directly applied to other neural-tube domains and species.

The hepatitis C virus (HCV) non-structural protein 3 (NS3) is essential for HCV maturation. The NS3/4A protease is a target for several HCV treatments and is a well-known target for HCV drug discovery. The protein is membrane associated and thus probably interacts with other membrane proteins. However, the vast majority of known NS3 host partners are soluble proteins rather than membrane proteins, most likely due to lack of appropriate platforms for their discovery. Utilization of an integrated microfluidics platform enables analysis of membrane proteins in their native form. We screened over 2800 membrane proteins for interaction with NS3 and 90 previously unknown interactions were identified. Of these, several proteins were selected for validation by co-immunoprecipitation and for NS3 proteolytic activity. Bearing in mind the considerable number of interactions formed, together with the popularity of NS3/4A protease as a drug target, it was striking to note its lack of proteolytic activity. Only a single protein, Neuregulin1, was observed to be cleaved, adding to the 3 known NS3/4A cleavage targets. Neuregulin1 participates in neural proliferation. Recent studies have shown its involvement in HCV infection and hepatocellular carcinoma. We showed that NS3/4A triggers an increase in neuregulin1 mRNA levels in HCV infected cells. Despite this increase, its protein concentration is decreased due to proteolytic cleavage. Additionally, its EGF-like domain levels were increased, possibly explaining the ErbB2 and EGFR upregulation in HCV infected cells. The newly discovered protein interactions may provide insights into HCV infection mechanisms and potentially provide new therapeutic targets against HCV. © 2018 Elsevier B.V.