@article {2979,
	title = {MAN1 and emerin have overlapping function(s) essential for chromosome segregation and cell division in Caenorhabditis elegans.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {100},
	year = {2003},
	month = {2003 Apr 15},
	pages = {4598-603},
	abstract = {Emerin and MAN1 are LEM domain-containing integral membrane proteins of the vertebrate nuclear envelope. The function of MAN1 is unknown, whereas emerin is known to interact with nuclear lamins, barrier-to-autointegration factor (BAF), nesprin-1 alpha, and a transcription repressor. Mutations in emerin cause X-linked recessive Emery-Dreifuss muscular dystrophy. Emerin and MAN1 homologs are both conserved in Caenorhabditis elegans, but loss of Ce-emerin has no detectable phenotype. We therefore used C. elegans to test the hypothesis that Ce-MAN1 overlaps functionally with Ce-emerin. Supporting this model, Ce-MAN1 interacted directly with Ce-lamin and Ce-BAF in vitro and required Ce-lamin for its nuclear envelope localization. Interestingly, RNA interference-mediated removal of approximately 90\% of Ce-MAN1 was lethal to approximately 15\% of embryos. However, in the absence of Ce-emerin, approximately 90\% reduction of Ce-MAN1 was lethal to all embryos by the 100-cell stage, with a phenotype involving repeated cycles of anaphase chromosome bridging and cytokinesis ["cell untimely torn" (cut) phenotype]. Immunostaining showed that the anaphase-bridged chromatin specifically retained a mitosis-specific phosphohistone H3 epitope and failed to recruit detectable Ce-lamin or Ce-BAF. These findings show that LEM domain proteins are essential for cell division and that Ce-emerin and Ce-MAN1 share at least one and possibly multiple overlapping functions, which may be relevant to Emery-Dreifuss muscular dystrophy.},
	keywords = {Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Division, Chromosome Segregation, DNA-Binding Proteins, Emery-Dreifuss, Genes, Helminth, Humans, Membrane Proteins, Muscular dystrophy, Mutation, Nuclear Proteins, Phenotype, RNA Interference, Thymopoietins},
	issn = {0027-8424},
	doi = {10.1073/pnas.0730821100},
	author = {Liu, Jun and Lee, Kenneth K and Segura-Totten, Miriam and Neufeld, Ester and Wilson, Katherine L and Gruenbaum, Yosef}
}
