@article {2933,
	title = {Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.},
	journal = {PLoS Genet},
	volume = {6},
	year = {2010},
	month = {2010 Nov},
	pages = {e1001219},
	abstract = {The Caenorhabditis elegans inner nuclear envelope protein matefin/SUN-1 plays a conserved, pivotal role in the process of genome haploidization. CHK-2-dependent phosphorylation of SUN-1 regulates homologous chromosome pairing and interhomolog recombination in Caenorhabditis elegans. Using time-lapse microscopy, we characterized the movement of matefin/SUN-1::GFP aggregates (the equivalent of chromosomal attachment plaques) and showed that the dynamics of matefin/SUN-1 aggregates remained unchanged throughout leptonene/zygotene, despite the progression of pairing. Movement of SUN-1 aggregates correlated with chromatin polarization. We also analyzed the requirements for the formation of movement-competent matefin/SUN-1 aggregates in the context of chromosome structure and found that chromosome axes were required to produce wild-type numbers of attachment plaques. Abrogation of synapsis led to a deceleration of SUN-1 aggregate movement. Analysis of matefin/SUN-1 in a double-strand break deficient mutant revealed that repair intermediates influenced matefin/SUN-1 aggregate dynamics. Investigation of movement in meiotic regulator mutants substantiated that proper orchestration of the meiotic program and effective repair of DNA double-strand breaks were necessary for the wild-type behavior of matefin/SUN-1 aggregates.},
	keywords = {Animals, Biological, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Cycle Proteins, Cell Nucleus, Chromatin, Chromosomes, Cytoplasmic and Nuclear, Cytoskeleton, DNA Breaks, Double-Stranded, GENOTYPE, Meiotic Prophase I, Mitosis, Models, Nuclear Proteins, protein structure, Protein Transport, Quaternary, Receptors, Synaptonemal Complex},
	issn = {1553-7404},
	doi = {10.1371/journal.pgen.1001219},
	author = {Baudrimont, Antoine and Penkner, Alexandra and Woglar, Alexander and Machacek, Thomas and Wegrostek, Christina and Gloggnitzer, Jiradet and Fridkin, Alexandra and Klein, Franz and Gruenbaum, Yosef and Pasierbek, Pawel and Jantsch, Verena}
}
