@article {17092,
	title = {Circadian mechanisms in murine and human bone marrow mesenchymal stem cells following dexamethasone exposure},
	journal = {Bone},
	volume = {42},
	number = {5},
	year = {2008},
	note = {Wu, Xiying Yu, Gang Parks, Helen Hebert, Teddi Goh, Brian C Dietrich, Marilyn A Pelled, Gadi Izadpanah, Reza Gazit, Dan Bunnell, Bruce A Gimble, Jeffrey M eng P30 DK072476-03/DK/NIDDK NIH HHS/ P30 DK072476/DK/NIDDK NIH HHS/ R21 DE016371/DE/NIDCR NIH HHS/ DE016371/DE/NIDCR NIH HHS/ DK072476/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov{\textquoteright}t 2008/02/28 09:00 Bone. 2008 May;42(5):861-70. doi: 10.1016/j.bone.2007.12.226. Epub 2008 Jan 26.},
	pages = {861-70},
	type = {Journal Article},
	abstract = {A core group of regulatory factors control circadian rhythms in mammalian cells. While the suprachiasmatic nucleus in the brain serves as the central core circadian oscillator, circadian clocks also exist within peripheral tissues and cells. A growing body of evidence has demonstrated that >20\% of expressed mRNAs in bone and adipose tissues oscillate in a circadian manner. The current manuscript reports evidence of the core circadian transcriptional apparatus within primary cultures of murine and human bone marrow-derived mesenchymal stem cells (BMSCs). Exposure of confluent, quiescent BMSCs to dexamethasone synchronized the oscillating expression of the mRNAs encoding the albumin D binding protein (dbp), brain-muscle arnt-like 1 (bmal1), period 3 (per3), rev-erb alpha (Rev A), and rev-erb beta (Rev B). The genes displayed a mean oscillatory period of 22.2 to 24.3 h. The acrophase or peak expression of mRNAs encoding "positive" (bmal1) and "negative" (per3) components of the circadian regulatory apparatus were out of phase with each other by approximately 8-12 h, consistent with in vivo observations. In vivo, phosphyrylation by glycogen synthase kinase 3beta (GSK3beta) is known to regulate the turnover of per3 and components of the core circadian regulatory apparatus. In vitro addition of lithium chloride, a GSK3beta inhibitor, significantly shifted the acrophase of all genes by 4.2-4.7 h oscillation in BMSCs; however, only the male murine BMSCs displayed a significant increase in the length of the period of oscillation. We conclude that human and murine BMSCs represent a valid in vitro model for the analysis of circadian mechanisms in bone metabolism and stem cell biology.},
	keywords = {ARNTL Transcription Factors Adult Animals Antigens, CD/analysis Basic Helix-Loop-Helix Transcription Factors/genetics Bone Marrow Cells/cytology/drug effects/metabolism Cell Cycle Proteins/genetics Circadian Rhythm/*physiology DNA-Binding Proteins/genetics Dexamethasone/*pharmacology Female Gene Expressio, Cytoplasmic and Nuclear/genetics Repressor Proteins/genetics Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/genetics, Group D, Inbred C57BL Nuclear Proteins/genetics Nuclear Receptor Subfamily 1, Member 1 Period Circadian Proteins Receptors},
	issn = {8756-3282 (Print) 1873-2763 (Linking)},
	doi = {10.1016/j.bone.2007.12.226},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/18302991},
	author = {Wu, X. and Yu, G. and Parks, H. and Hebert, T. and Goh, B. C. and Dietrich, M. A. and Pelled, G. and Izadpanah, R. and Gazit, D. and Bunnell, B. A. and Gimble, J. M.}
}
