@article {17093,
	title = {Bioluminescent imaging in bone},
	journal = {Methods Mol Biol},
	volume = {455},
	year = {2008},
	note = {Zilberman, Yoram Gafni, Yossi Pelled, Gadi Gazit, Zulma Gazit, Dan eng 2008/05/09 09:00 Methods Mol Biol. 2008;455:261-72. doi: 10.1007/978-1-59745-104-8_18.},
	pages = {261-72},
	type = {Journal Article},
	abstract = {Monitoring gene expression in vitro and in vivo, is crucial when analyzing osteogenesis and developing effective bone gene therapy protocols. Until recently, molecular analytical tools were only able to detect protein expression either in vitro or in vivo. These systems include histology and immunohistochemistry, fluorescent imaging, PET (micro-PET), CT (micro-CT), and bioluminescent imaging. The last is the only system to date that can enable efficient quantitative monitoring of gene expression both in vitro and in vivo. Effective bioluminescent imaging in bone can be achieved by using transgenic mice harboring the luciferase reporter gene, downstream of an osteogenesis specific promoter. The aim of this chapter is to comprehensively describe the various protocols needed for the detection of bioluminescence in bone development and repair.},
	keywords = {Animals Bone and Bones/*cytology/metabolism Humans Immunohistochemistry/methods Luminescent Proteins/genetics/*metabolism Mice Mice, Fluorescence/instrumentation/methods Osteocalcin/genetics Recombinant Fusion Proteins/genetics/metabolism, Transgenic *Microscopy},
	issn = {1064-3745 (Print) 1064-3745 (Linking)},
	doi = {10.1007/978-1-59745-104-8_18},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/18463824},
	author = {Zilberman, Y. and Gafni, Y. and Pelled, G. and Gazit, Z. and Gazit, D.}
}
