@article {17130,
	title = {Transient overexpression of Ppargamma2 and C/ebpalpha in mesenchymal stem cells induces brown adipose tissue formation},
	journal = {Regen Med},
	volume = {8},
	number = {3},
	year = {2013},
	note = {Sheyn, Dmitriy Pelled, Gadi Tawackoli, Wafa Su, Susan Ben-David, Shiran Gazit, Dan Gazit, Zulma eng Research Support, Non-U.S. Gov{\textquoteright}t England 2013/05/01 06:00 Regen Med. 2013 May;8(3):295-308. doi: 10.2217/rme.13.25.},
	pages = {295-308},
	type = {Journal Article},
	abstract = {BACKGROUND: Brown adipose tissue plays a pivotal role in mammal metabolism and thermogenesis. It has a great therapeutic potential in several metabolic disorders such as obesity and diabetes. Mesenchymal stem cells (MSCs) are suitable candidates for brown adipose tissue formation de novo. Ppargamma2 and C/ebpalpha are nucleic receptors known to mediate adipogenic differentiation. We hypothesized that overexpression of the Ppargamma2 and C/ebpalpha genes in MSCs would lead to the formation of adipose tissue. MATERIALS \& METHODS: MSCs bearing the Luc reporter gene were transfected to overexpress Ppargamma2 and C/ebpalpha. Differentiation of nucleofected cells was evaluated in vitro and in vivo following ectopic implantation of the cells in C3H/HeN mice. RESULTS: After implantation, the engineered cells survived for 5 weeks and brown adipose-like tissue was observed in histological samples. Immunostaining and bioluminescent imaging showed new adipocytes expressing Luc and the brown adipose tissue marker, UCP1, in vitro and in vivo. CONCLUSION: We show that gene delivery of transcription factors into MSCs generates brown adipose tissue in vitro and in vivo.},
	keywords = {Adipogenesis/genetics Adipose Tissue, Brown/*metabolism Animals Biomarkers/metabolism CCAAT-Enhancer-Binding Protein-alpha/*metabolism Cell Differentiation/genetics Cell Line Cell Survival/genetics Gene Expression Profiling Genetic Engineering HEK293 Cells Humans Ion Channels/metabolism Lumi},
	issn = {1746-076X (Electronic) 1746-0751 (Linking)},
	doi = {10.2217/rme.13.25},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/23627824},
	author = {Sheyn, D. and Pelled, G. and Tawackoli, W. and Su, S. and Ben-David, S. and Gazit, D. and Gazit, Z.}
}
