@article {728e0a52af144d68af2329fdf5544d5c,
	title = {Human antibodies targeting a Mycobacterium transporter protein mediate protection against tuberculosis},
	journal = {Nature Communications},
	volume = {12},
	number = {1},
	year = {2021},
	note = {Publisher Copyright: {\textcopyright} 2021, The Author(s).},
	month = {dec},
	publisher = {Nature Publishing Group},
	abstract = {Mycobacterium tuberculosis (Mtb) exposure drives antibody responses, but whether patients with active tuberculosis elicit protective antibodies, and against which antigens, is still unclear. Here we generate monoclonal antibodies from memory B cells of one patient to investigate the B cell responses during active infection. The antibodies, members of four distinct B cell clones, are directed against the Mtb phosphate transporter subunit PstS1. Antibodies p4-36 and p4-163 reduce Mycobacterium bovis-BCG and Mtb levels in an ex vivo human whole blood growth inhibition assay in an FcR-dependent manner; meanwhile, germline versions of p4-36 and p4-163 do not bind Mtb. Crystal structures of p4-36 and p4-170, complexed to PstS1, are determined at 2.1 {\r A} and 2.4 {\r A} resolution, respectively, to reveal two distinctive PstS1~epitopes. Lastly, a prophylactic p4-36 and p4-163 treatment in Mtb-infected Balb/c mice reduces bacterial lung burden by 50\%. Our study shows that inhibitory anti-PstS1 B cell responses arise during active tuberculosis.},
	issn = {2041-1723},
	doi = {10.1038/s41467-021-20930-0},
	author = {Avia Watson and Hao Li and Bingting Ma and Ronen Weiss and Daniele Bendayan and Lilach Abramovitz and Noam Ben-Shalom and Michael Mor and Erica Pinko and Bar Oz, Michal and Zhenqi Wang and Fengjiao Du and Yu Lu and Jan Rybniker and Rony Dahan and Hairong Huang and Daniel Barkan and Ye Xiang and Babak Javid and Freund, Natalia T.}
}
