@article {3041253,
	title = {ACTIBIND, a T2 RNase, competes with angiogenin and inhibits human melanoma growth, angiogenesis, and metastasis},
	journal = {Cancer Res},
	volume = {67},
	year = {2007},
	note = {Schwartz, Betty Shoseyov, Oded Melnikova, Vladislava O McCarty, Marya Leslie, Michael Roiz, Levava Smirnoff, Patricia Hu, Guo-Fu Lev, Dina Bar-Eli, Menashe eng CA76098/CA/NCI NIH HHS/ P50CA093459/CA/NCI NIH HHS/ Research Support, N.I.H., Extramural Cancer Res. 2007 Jun 1;67(11):5258-66. doi: 10.1158/0008-5472.CAN-07-0129.},
	pages = {5258-66},
	type = {Journal Article},
	abstract = {Melanoma is a very aggressive and highly angiogenic tumor in which standard treatments have had only limited success. Patients with advanced disease have a 5-year survival rate of 5\%. In search for alternatives, we identified a natural product extracted from the fungus Aspergillus niger, termed ACTIBIND, that inhibits tumor growth and metastasis of melanoma in vivo. ACTIBIND, a T2 RNase, exerts antitumorigenic and antiangiogenic activities by competing with the angiogenic factor angiogenin (itself an RNase homologue). Thus, there was decreased expression and activity of the matrix metalloproteinase 2 in melanoma and vascular endothelial cells, decreased vascularization, and increased tumor cell apoptosis in vivo. ACTIBIND significantly inhibited angiogenesis in an in vivo angiogenesis assay with sponges containing angiogenin. In vitro, ACTIBIND was internalized by both melanoma and human umbilical vein endothelial cells, reached the cell nuclei, and inhibited the activity of angiogenin response elements in a dose-dependent manner. Collectively, our data indicate that ACTIBIND should be tested for its potential as a new antiangiogenic modality for the treatment of melanoma.},
	keywords = {Animals Apoptosis/drug effects Aspergillus niger/enzymology Cell Growth Processes/drug effects Cell Line, Genetic, Inbred BALB C Mice, Nude Neoplasm Metastasis Neovascularization, Pancreatic/antagonists \& inhibitors/metabolism/*pharmacology Transcription, Pathologic/drug therapy/pathology Ribonuclease, Tumor Drug Interactions Endoribonucleases/metabolism/*pharmacology Endothelial Cells/drug effects/metabolism/pathology Humans Luciferases/metabolism Male Matrix Metalloproteinase 2/biosynthesis/metabolism Matrix Metalloproteinase Inhibitors Melanoma/bloo},
	issn = {0008-5472 (Print) 0008-5472 (Linking)},
	doi = {10.1158/0008-5472.CAN-07-0129},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/17545605},
	author = {Schwartz, B. and Shoseyov, O. and Melnikova, V.O. and McCarty, M. and Leslie, M. and Roiz, L. and Smirnoff, P. and Hu, G. F. and Lev, D. and Bar-Eli, M.}
}
