@article {3041230,
	title = {Recombinant ostreolysin induces brown fat-like phenotype in HIB-1B cells},
	journal = {Mol Nutr Food Res},
	volume = {61},
	year = {2017},
	note = {Oren, Tom Nimri, Lili Yehuda-Shnaidman, Einav Staikin, Katy Hadar, Yitzhak Friedler, Assaf Amartely, Hadar Slutzki, Michal Pizio, Antonella Di Niv, Masha Y Peri, Irena Graeve, Lutz Schwartz, Betty eng Research Support, Non-U.S. Gov{\textquoteright}t Germany Mol Nutr Food Res. 2017 Sep;61(9). doi: 10.1002/mnfr.201700057. Epub 2017 Jun 9.},
	type = {Journal Article},
	abstract = {SCOPE: Brown adipose tissue (BAT) is the main regulator of thermogenesis by increasing energy expenditure through the uncoupling of oxidative metabolism from ATP synthesis. There is a growing body of evidence for BAT being the key responsible organ in combating obesity and its related disorders. Herein we propose the fungal protein ostreolysin (Oly), which has been previously shown to bind to cholesterol-enriched raft-like membrane domains (lipid rafts) of mammalian cells, as a suitable candidate for interaction with brown preadipocytes. The aim of the present study was therefore to characterize the mechanism by which a recombinant version of ostreolysin (rOly) induces brown adipocyte differentiation. METHODS AND RESULTS: Primary isolated brown preadipocytes or HIB-1B brown preadipocyte cells were treated with rOly and the effects on morphology, lipid accumulation, respiration rate, and associated gene and protein expression were measured. rOly upregulated mRNA and protein levels of factors related to brown adipocyte differentiation, induced lipid droplet formation, and increased cellular respiration rate due to expression of uncoupling protein 1. rOly also upregulated beta-tubulin expression, and therefore microtubules might be involved in its mechanism of action. CONCLUSION: rOly promotes brown adipocyte differentiation, suggesting a new mechanism for rOly{\textquoteright}s contribution to the battle against obesity.},
	keywords = {Adipocytes, Brown/cytology/*drug effects/metabolism Animals CCAAT-Enhancer-Binding Proteins/genetics Cell Differentiation/drug effects Fungal Proteins/chemistry/metabolism/pharmacology Gene Expression Regulation/drug effects Hemolysin Proteins/chemistry/metabolism/*, Secondary Recombinant Proteins/pharmacology Tubulin/chemistry Uncoupling Protein 1/genetics *Brown adipocyte differentiation *Brown adipose tissue *Lipid droplet *Obesity *Ostreolysin},
	issn = {1613-4133 (Electronic) 1613-4125 (Linking)},
	doi = {10.1002/mnfr.201700057},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/28464422},
	author = {Oren, T. and Nimri, L. and Yehuda-Shnaidman, E. and Staikin, K. and Hadar, Y. and A. Friedler and Amartely, H. and Slutzki, M. and Pizio, A.D. and Niv, M.Y. and Peri, I. and Graeve, L. and Schwartz, B.}
}
