@article {3041225,
	title = {Ostreolysin induces browning of adipocytes and ameliorates hepatic steatosis},
	journal = {J Gastroenterol Hepatol},
	volume = {33},
	year = {2018},
	note = {Nimri, Lili Staikin, Katerina Peri, Irena Yehuda-Shnaidman, Einav Schwartz, Betty eng 0390093/Chief Scientist Office/United Kingdom 0390093/Nofar, Chief Scientist Australia J Gastroenterol Hepatol. 2018 Dec;33(12):1990-2000. doi: 10.1111/jgh.14259. Epub 2018 May 15.},
	pages = {1990-2000},
	type = {Journal Article},
	abstract = {BACKGROUND AND AIM: Non-alcoholic fatty liver disease (NAFLD) is associated with all features of the metabolic syndrome. Deposition of excess triglycerides in liver cells, a hallmark of NAFLD, is associated with loss of insulin sensitivity. Ostreolysin (Oly) is a 15-kDa fungal protein known to interact with cholesterol-enriched raft-like membrane domains. We aim to test whether a recombinant version of Oly (rOly) can induce functional changes in vitro in adipocytes or in vivo in mice fed a high-fat diet (HFD). METHODS: White preadipocyte 3T3-L1 cells or mouse primary adipocytes treated with rOly. Male C57BL/6 mice were fed a control or HFD and treated with saline or with rOly (1 mg/kg BW) every other day for 4 weeks. RESULTS: White preadipocyte 3T3-L1 cells or mouse primary adipocytes treated with rOly acquire a browning phenotype through activation of 5{\textquoteright} adenosine monophosphate-activated protein kinase and downregulation of tumor necrosis factor alpha-mediated activation of IkappaB kinase epsilon and TANK-binding kinase 1. HFD-fed mice treated with rOly showed a 10\% reduction in BW and improved glucose tolerance, which paralleled improved expression of liver and adipose functionality, metabolism, and inflammation status, mimicking the in vitro findings. CONCLUSION: This study provides first evidence of rOly{\textquoteright}s prevention of HFD-induced NAFLD by stimulating liver and adipose muscle tissue functionality and oxidative potential, improving glucose tolerance, and ameliorating the metabolic profile of diet-induced obese mice.},
	keywords = {3T3-L1 Cells AMP-Activated Protein Kinases/metabolism Adipocytes, Animal Enzyme Activation Fungal Proteins/pharmacology Hemolysin Proteins/*pharmacology I-kappa B Kinase/metabolism Liver/*drug effects/metabolism/pathology Male Mice Mice, Brown/*drug effects/metabolism/pathology Adipocytes, High-Fat Disease Models, Inbred C57BL Non-alcoholic Fatty Liver Disease/metabolism/pathology/*prevention \& control Phenotype Protein-Serine-Threonine Kinases/metabolism Signal Transduction/drug effects browning inflammation non-alcoholic fatty liver disease ostreolysin steatosis, White/*drug effects/metabolism/pathology Adiposity/drug effects Animals Blood Glucose/drug effects/metabolism Diet},
	issn = {1440-1746 (Electronic) 0815-9319 (Linking)},
	doi = {10.1111/jgh.14259},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/29663549},
	author = {Nimri, L. and Staikin, K. and Peri, I. and Yehuda-Shnaidman, E. and Schwartz, B.}
}
