@article {3041208,
	title = {Glucans from the edible mushroom Pleurotus pulmonarius inhibit colitis-associated colon carcinogenesis in mice},
	journal = {J Gastroenterol},
	volume = {47},
	year = {2012},
	note = {Lavi, Iris Nimri, Lili Levinson, Dana Peri, Irena Hadar, Yitzhak Schwartz, Betty eng Research Support, Non-U.S. Gov{\textquoteright}t Japan J Gastroenterol. 2012 May;47(5):504-18. doi: 10.1007/s00535-011-0514-7. Epub 2011 Dec 21.},
	pages = {504-18},
	type = {Journal Article},
	abstract = {BACKGROUND: We have recently demonstrated that polysaccharides from fruiting body extract (FBE) or mycelia extract (ME) of the edible mushroom Pleurotus pulmonarius exert antiproliferative effects in intestinal cells and an anti-inflammatory effect in a dextran sulfate sodium (DSS) mouse model of acute colitis. The aim of this study was to assess the role of fungal FBE and ME in colon carcinogenesis. METHODS: In vitro, human colorectal cancer cells were treated with FBE and ME and analyzed for inflammation response, for markers of apoptosis, and for cell-cycle progression. In vivo, FBE and ME were tested in a mouse model of colitis-associated colorectal carcinogenesis induced by cyclic treatments with DSS and azoxymethane. Treated mice were fed a daily diet containing 2 or 20 mg FBE or ME per mouse for 80 days. RESULTS: In vitro, FBE and ME induced apoptosis in a dose-responsive manner and modulated the expression of Bcl-2, Bax, and cytochrome c, and blocked tumor necrosis factor (TNF)-alpha-induced inhibitor of nuclear factor (NF) (Ikappa)-Balpha degradation and NF-kappaB nuclear translocation. In vivo, dietary administration of FBE and ME significantly reduced the formation of aberrant crypt foci, which precedes colorectal cancer, and of microadenomas. The treatments significantly lowered the expression of proliferating cell nuclear antigen and increased the number of cells undergoing apoptosis in the colon. Additionally, FBE and ME inhibited the expression of the proinflammatory cytokine TNF-alpha in colonic tissue. CONCLUSIONS: We conclude that P. pulmonarius FBE and ME inhibit colitis-associated colon carcinogenesis induced in mice through the modulation of cell proliferation, induction of apoptosis, and inhibition of inflammation.},
	keywords = {Animals Apoptosis/drug effects Cell Cycle/drug effects Cell Transformation, Antitumor/methods Fruiting Bodies, Cultured, Drug Drug Screening Assays, Fungal/chemistry Glucans/pharmacology/*therapeutic use Humans Mice Mice, Inbred Strains Mycelium/chemistry Phytotherapy/*methods Plant Extracts/pharmacology/therapeutic use Pleurotus/*chemistry Tumor Cells, Neoplastic/*drug effects/metabolism/pathology Colitis/complications/*drug therapy/pathology Colorectal Neoplasms/etiology/metabolism/pathology/*prevention \& control Dose-Response Relationship},
	issn = {1435-5922 (Electronic) 0944-1174 (Linking)},
	doi = {10.1007/s00535-011-0514-7},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/22187166},
	author = {Lavi, I. and Nimri, L. and Levinson, D. and Peri, I. and Hadar, Y. and Schwartz, B.}
}
