@article {3041185,
	title = {The ion conductances of colonic crypts from dexamethasone-treated rats},
	journal = {Pflugers Arch},
	volume = {431},
	year = {1996},
	note = {Ecke, D Bleich, M Schwartz, B Fraser, G Greger, R eng Research Support, Non-U.S. Gov{\textquoteright}t Germany 1996/01/01 00:00 Pflugers Arch. 1996 Jan;431(3):419-26. doi: 10.1007/BF02207281.},
	pages = {419-26},
	type = {Journal Article},
	abstract = {{Whole-cell patch-clamp studies were performed in isolated colonic crypts of rats pretreated with dexamethasone (6 mg/kg subcutaneously on 3 days consecutively prior to the experiment). The cells were divided into three categories according to their position along the crypt axis: surface cells (s.c.); mid-crypt cells (m.c.) and crypt base cells (b.c.). The zero-current membrane voltage (Vm) was -56 +/- 2 mV in s.c (n = 34); -76 +/- 2 mV in m.c. (n = 47); and -87 +/- 1 mV in b.c. (n = 87). The whole-cell conductance (Gm) was similar (8-12 nS) in all three types of cells. A fractional K+ conductance accounting for 29-67\% of Gm was present in all cell types. A Na+ conductance was demonstrable in s.c. by the hyperpolarizing effect on Vm of a low-Na+ (5 mmol/l) solution. In m.c. and b.c. the hyperpolarizing effect was much smaller, albeit significant. Amiloride had a concentration-dependent hyperpolarizing effect on Vm in m.c. and even more so in s.c.. It reduced Gm by approximately 12\%. The dissociation constant (KD) was around 0.2 micromol/l. Triamterene had a comparable but not additive effect (KD = 30 micromol/l},
	keywords = {Amiloride/pharmacology Animals Chlorides/metabolism Colon/cytology/drug effects/physiology Cyclic AMP/pharmacology Dexamethasone/administration \& dosage/*pharmacology Electric Conductivity Electrophysiology In Vitro Techniques Injections, Subcutaneous Intestinal Absorption Patch-Clamp Techniques Potassium/physiology Rats Sodium/*pharmacokinetics Triamterene/pharmacology},
	issn = {0031-6768 (Print) 0031-6768 (Linking)},
	doi = {10.1007/BF02207281},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/8584437},
	author = {Ecke, D. and Bleich, M. and Schwartz, B. and Fraser, G. and Greger, R.}
}
