Binding assay and preliminary X-ray crystallographic analysis of ACTIBIND, a protein with anticarcinogenic and antiangiogenic activities

Citation:

M. De Leeuw, Roiz, L. , Smirnoff, P. , Schwartz, B. , Shoseyov, O. , and Almog, O. . 2007. “Binding Assay And Preliminary X-Ray Crystallographic Analysis Of Actibind, A Protein With Anticarcinogenic And Antiangiogenic Activities”. Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, Pt 8, Pp. 716-9. doi:10.1107/S1744309107034483.

Abstract:

{ACTIBIND is a T2 RNase extracellular glycoprotein produced by the mould Aspergillus niger B1 (CMI CC 324626) that possesses anticarcinogenic and antiangiogenic activities. ACTIBIND was found to be an actin-binding protein that interacts with rabbit muscle actin in a 1:2 molar ratio (ACTIBIND:actin) with a binding constant of 16.17 x 10(4) M(-1). Autoclave-treated ACTIBIND (EI-ACTIBIND) lost its RNase activity, but its actin-binding ability was conserved. ACTIBIND crystals were grown using 20% PEG 3350, 0.2 M ammonium dihydrogen phosphate solution at room temperature (293 K). One to four single crystals appeared in each droplet within a few days and grew to approximate dimensions of 0.5 x 0.5 x 0.5 mm after about two weeks. Diffraction studies of these crystals at low temperature (100 K) indicated that they belong to the P3(1)21 space group, with unit-cell parameters a = 78

Notes:

de Leeuw, Marina Roiz, Levava Smirnoff, Patricia Schwartz, Betty Shoseyov, Oded Almog, Orna eng England Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt 8):716-9. doi: 10.1107/S1744309107034483. Epub 2007 Jul 28.