פרסומים

Tungsten, an essential refractory metal material, has the characteristics of high melting and boiling points, high hardness, low expansion coefficient, and low vapor pressure. An indirect strategy to print three-dimensional (3D) refractory metal materials via digital light processing (DLP) followed by a post-treatment process was proposed. To analyze this strategy, a transparent ink with tungsten salts was developed, printed into a 3D precursor via DLP, and subsequently transited into 3D porous tungsten. The ultraviolet rheological properties and stability of the ink, transition process from the precursor to a 3D article, and the properties of the obtained 3D porous tungsten were investigated. This ink was preferable for DLP 3D printing, possessing consistency, stability and favorable absorbance at the wavelength of 385 nm. With increasing temperature, the weight of the tungsten salt in the 3D precursor decreased by 8.97% and was transited to tungsten oxide below 460 °C, reduced to pure nano-sized tungsten at approximately 700 °C, and finally sintered into porous articles. The organics initially contributed to polymerization during printing as well as reduction as a carbon reducer after pyrolysis. The pore size distribution of porous tungsten is nonlinear or multimodal, depending on the final sintering temperature. At 1200 °C, two distinct peaks are observed in the pore distribution curves of the products. At 1400 °C, the small pore as a whole decreases from approximately 100-1000 nm. Correspondingly, the relative density of the samples increased with temperature.

Timbre is an integral dimension of musical sound quality, and people accumulate knowledge about timbre of sounds generated by various musical instruments throughout their life. Recent studies have proposed the possibility that musical sound is crossmodally integrated with visual information related to the sound. However, little is known about the influence of visual information on musical timbre perception. The present study investigated the automaticity of crossmodal integration between musical timbre and visual image of hands playing musical instruments. In the experiment, an image of hands playing piano or violin, or a control scrambled image was presented to participants unconsciously. Simultaneously, participants heard intermediate sounds synthesised by morphing piano and violin sounds with the same note. The participants answered whether the musical tone sounded like piano or violin. The results revealed that participants were more likely to perceive violin sound when an image of a violin was presented unconsciously than when playing piano was presented. This finding indicates that timbral perception of musical sound is influenced by visual information of musical performance without conscious awareness, supporting the automaticity of crossmodal integration in musical timbre perception.

Research conducted on emotionality in bilinguals suggests that language use modulates emotional expression. The current study examines bilingual disadvantaged minority members’ emotional experience and expression as shaped by the group relations in a conflict area. We hypothesised that, in general, greater emotionality will be found in one’s native language. Moreover, since the second language is imposed and acquired in a negative context, there may be differential effects on negative and positive language. A novel ecological paradigm was used: Twenty-eight Palestinian citizens of Israel were videotaped while recounting emotional stories in both Arabic (L1) and Hebrew (L2), resulting in 212 videos. Two studies followed: In Study 1 we compared participants’ emotional ratings (1a) and analyzed the content of emotional expression (1b). In Study 2, American participants rated emotional expressiveness. In Study 1, an interaction effect was found between language and valence, with less positive emotions and expressions in L2. In Study 2, a general difference in expressiveness was found in favour of L1. These studies show an effect of power disparities on the emotional load of the second language, thus highlighting the emotional costs of using a second language acquired in a conflict area.

Background: COVID-19 has had a disproportionate and unprecedented impact on children with disabilities, their parents and families. This impact has been particularly evident during periods of lockdowns and severe restrictions. Objective: This study employed the social model of disability to illuminate negative and positive experiences of Israeli parents of children with disabilities during the first COVID-19 lockdown, as well as the way social environments, particularly educational and welfare services, shape that experience. Methods: The study draws upon thematic analysis of written responses of 80 Israeli parents to open-ended questions. Results: Three main themes were found: (1) lack of responsiveness of both informal and formal supports to family needs, mainly in the educational and welfare systems; (2) juggling multiple roles and tasks along with the parents' routinely intensive caregiving role; and (3) the impact on the family's wellbeing and relationships. Conclusion: These results are discussed in light of the social model of disability, showing how social restrictions and barriers affect both negative and positive experiences of these families. Also discussed are practical implications for future crises.

Background: COVID-19 has had a disproportionate and unprecedented impact on children with disabilities, their parents and families. This impact has been particularly evident during periods of lockdowns and severe restrictions. Objective: This study employed the social model of disability to illuminate negative and positive experiences of Israeli parents of children with disabilities during the first COVID-19 lockdown, as well as the way social environments, particularly educational and welfare services, shape that experience. Methods: The study draws upon thematic analysis of written responses of 80 Israeli parents to open-ended questions. Results: Three main themes were found: (1) lack of responsiveness of both informal and formal supports to family needs, mainly in the educational and welfare systems; (2) juggling multiple roles and tasks along with the parents' routinely intensive caregiving role; and (3) the impact on the family's wellbeing and relationships. Conclusion: These results are discussed in light of the social model of disability, showing how social restrictions and barriers affect both negative and positive experiences of these families. Also discussed are practical implications for future crises.

The plant hormone abscisic acid (ABA) plays a central role in the regulation of seed maturation and dormancy. ABA also restrains germination under abiotic-stress conditions. Here, we show in tomato (Solanum lycopersicum) that the ABA importer ABA-IMPORTING TRANSPORTER 1.1 (AIT1.1/NPF4.6) has a role in radicle emergence under salinity conditions. AIT1.1 expression was upregulated following seed imbibition, and CRISPR/Cas9-derived ait1.1 mutants exhibited faster radicle emergence, increased germination and partial resistance to ABA. AIT1.1 was highly expressed in the endosperm, but not in the embryo, and ait1.1 isolated embryos did not show resistance to ABA. On the other hand, loss of AIT1.1 activity promoted the expression of endosperm-weakening-related genes, and seed-coat scarification eliminated the promoting effect of ait1.1 on radicle emergence. Therefore, we propose that imbibition-induced AIT1.1 expression in the micropylar endosperm mediates ABA-uptake into micropylar cells to restrain endosperm weakening. While salinity conditions strongly inhibited wild-type M82 seed germination, high salinity had a much weaker effect on ait1.1 germination. We suggest that AIT1.1 evolved to inhibit germination under unfavorable conditions, such as salinity. Unlike other ABA mutants, ait1.1 exhibited normal seed longevity, and therefore, the ait1.1 allele may be exploited to improve seed germination in crops.

The tongue is a unique muscular organ situated in the oral cavity where it is involved in taste sensation, mastication, and articulation. As a barrier organ, which is constantly exposed to environmental pathogens, the tongue is expected to host an immune cell network ensuring local immune defence. However, the composition and the transcriptional landscape of the tongue immune system are currently not completely defined. Here, we characterised the tissue-resident immune compartment of the murine tongue during development, health and disease, combining single-cell RNA-sequencing with in situ immunophenotyping. We identified distinct local immune cell populations and described two specific subsets of tongue-resident macrophages occupying discrete anatomical niches. Cx3cr1+ macrophages were located specifically in the highly innervated lamina propria beneath the tongue epidermis and at times in close proximity to fungiform papillae. Folr2+ macrophages were detected in deeper muscular tissue. In silico analysis indicated that the two macrophage subsets originate from a common proliferative precursor during early postnatal development and responded differently to systemic LPS in vivo. Our description of the under-investigated tongue immune system sets a starting point to facilitate research on tongue immune-physiology and pathology including cancer and taste disorders.

DNA replication is a complex process tightly regulated to ensure faithful genome duplication, and its perturbation leads to DNA damage and genomic instability. Replication stress is commonly associated with slow and stalled replication forks. Recently, accelerated replication has emerged as a non-canonical form of replication stress. However, the molecular basis underlying fork acceleration is largely unknown. Here, we show that mutated HRAS activation leads to increased topoisomerase 1 (TOP1) expression, causing aberrant replication fork acceleration and DNA damage by decreasing RNA-DNA hybrids or R-loops. In these cells, restoration of TOP1 expression or mild replication inhibition rescues the perturbed replication and reduces DNA damage. Furthermore, TOP1 or RNaseH1 overexpression induces accelerated replication and DNA damage, highlighting the importance of TOP1 equilibrium in regulating R-loop homeostasis to ensure faithful DNA replication and genome integrity. Altogether, our results dissect a mechanism of oncogene-induced DNA damage by aberrant replication fork acceleration.

BACKGROUND: Endoscopic insufflation, long performed using air, is being replaced by carbon dioxide (CO2) at many pediatric centers, despite limited published data on its use in children. We have previously demonstrated that CO2 use during esophagogastroduodenoscopy (EGD) in non-intubated children is associated with transient elevations of end-tidal CO2 (EtCO2). This observation raised concerns about possible CO2 inhalation and systemic absorption. Here, we investigate this concern by concurrently measuring both EtCO2 and transcutaneous CO2 (tCO2) during upper endoscopic procedures in children. AIM: To determine if elevations in EtCO2 levels seen in non-intubated children undergoing CO2 insufflation during EGD are associated with elevated systemic CO2 levels. METHODS: Double-blinded, prospective, randomized clinical trial. Children were randomized 1:1 to receive either CO2 or air for endoscopic insufflation. EtCO2 was sampled with a CO2-sampling nasal cannula and tCO2 was monitored using the Radiometer transcutaneous monitoring device. RESULTS: Fifty nine patients were enrolled; 30 patients in the CO2 insufflation group and 29 in the air group. All patients underwent a procedure involving an EGD. Transient elevations in EtCO2 (defined as >60 mmHg) were observed only in the CO2 insufflation group. This contrasted with the similar elevations of tCO2 between the CO2 and air insufflation groups. None of these events were of clinically significant magnitude or duration. CONCLUSION: This study demonstrates that the observed transient elevations in EtCO2 seen during EGD in non-intubated children receiving CO2 insufflation are most likely measurements of eructated CO2 without evidence of excessive systemic absorption of CO2.

Optimizing glycosylation conditions for automated glycan assembly is highly challenging, demand wasteful use of precious building blocks and rely on nontrivial analyses. We developed a semi-quantitative method for automated optimization of glycosylation temperature that utilized minute quantities of donors and translated those conditions to solid-phase glycan assembly.