פרסומים

The intimate association between nuclear lamins and chromatin is thought to regulate higher order chromatin organization. Previous studies have mapped a region between the rod domain and the Ig fold in the tail domain of Drosophila melanogaster lamin Dm0, which binds chromatin in vitro via the histone H2A/H2B dimer. This region contains an evolutionarily conserved nuclear localization signal (NLS) KRKR, and a sequence composed of the amino acids TRAT. Here we show that binding of lamin Dm0 to chromatin requires both NLS and TRAT sequences. Substituting either of the threonine residues in the TRAT sequence with negatively charged residues decreases the binding of lamin Dm0 to chromatin, indicating that this binding could be regulated by phosphorylation. Both lamin Dm0 and C. elegans Ce-lamin bind directly to histone H2A in vitro and this binding requires the NLS. The amino and carboxyl tail domains of histone H2A are each essential, but not sufficient, for binding to lamin Dm0; only a polypeptide containing both histone H2A tail domains binds efficiently to lamin Dm0. Taken together, these results suggest that specific residues in lamin Dm0 and histone H2A mediate the attachment of the nuclear lamina to chromosomes in vivo, which could have implications on the understanding of laminopathic diseases.

Electrically controlled Bragg gratings implemented by periodic striations that were produced during the crystal growth are demonstrated in potassium lithium tantalate niobate crystals. The striations were generated by blowing air with periodic flow at the flux surface. The gratings were investigated by measurements of the diffraction efficiency versus the applied electric field. It was found that the composition grating induced correlated gratings of the refractive index and the low frequency dielectric constant. The latter, under the application of a uniform electric field, produced an electrically controlled birefringence grating through the quadratic electro-optic effect. [ABSTRACT FROM AUTHOR]Copyright of Applied Physics Letters is the property of American Institute of Physics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder’s express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This note revisits the problem of selection bias, using a simple binomial example. It focuses on selection that is introduced by observing the data and making decisions prior to formal statistical analysis. Decision rules and interpretation of confidence measure and results must then be taken relative to the point of view of the decision maker, i.e., before selection or after it. Such a distinction is important since inference can be considerably altered when the decision maker's point of view changes. This note demonstrates the issue, using both the frequentist and the Bayesian paradigms.

This work studies the value of two-person zero-sum repeated games in which at least one of the players is restricted to (mixtures of) bounded recall strategies. A (pure) k-recall strategy is a strategy that relies only on the last k periods of history. This work improves previous results [Lehrer, Neyman and Okada] on repeated games with bounded recall. We provide an explicit formula for the asymptotic value of the repeated game as a function of the stage game, the duration of the repeated game, and the recall of the agents.

Stem cell-based gene therapy and tissue engineering have been shown to be an efficient method for the regeneration of critical-sized bone defects. Despite being an area of active research over the last decade, no knowledge of the intrinsic ultrastructural and nanomechanical properties of such bone tissue exists. In this study, we report the nanomechanical properties of engineered bone tissue derived from genetically modified mesenchymal stem cells (MSCs) overexpressing the rhBMP2 gene, grown in vivo in the thigh muscle of immunocompetent mice for 4 weeks, compared to femoral bone adjacent to the transplantation site. The two types of bone had similar mineral contents (61 and 65 wt% for engineered and femoral bone, respectively), overall microstructures showing lacunae and canaliculi (both measured by back-scattered electron microscopy), chemical compositions (measured by energy dispersive X-ray analysis), and nanoscale topographical morphologies (measured by tapping-mode atomic force microscopy imaging or TMAFM). Nanoindentation experiments revealed that the small length scale mechanical properties were statistically different with the femoral bone (indented parallel to the bone long axis) being stiffer and harder (apparent elastic modulus, E approximately 27.3+/-10.5 GPa and hardness, H approximately 1.0+/-0.7G Pa) than the genetically engineered bone (E approximately 19.8+/-5.6 GPa, H approximately 0.9+/-0.4G Pa). TMAFM imaging showed clear residual indents characteristic of viscoelastic plastic deformation for both types of bone. However, fine differences in the residual indent area (smaller for the engineered bone), pile up (smaller for the engineered bone), and fracture mechanisms (microcracks for the engineered bone) were observed with the genetically engineered bone behaving more brittle than the femoral control.

The presence of live periosteal progenitor cells on the surface of bone autografts confers better healing than devitalized allograft. We have previously demonstrated in a murine 4 mm segmental femoral bone-grafting model that live periosteum produces robust endochondral and intramembraneous bone formation that is essential for effective healing and neovascularization of structural bone grafts. To the end of engineering a live pseudo-periosteum that could induce a similar response onto devitalized bone allograft, we seeded a mesenchymal stem cell line stably transfected with human bone morphogenic protein-2/beta-galactosidase (C9) onto devitalized bone allografts or onto a membranous small intestinal submucosa scaffold that was wrapped around the allograft. Histology showed that C9-coated allografts displayed early cartilaginous tissue formation at day 7. By 6 and 9 weeks, a new cortical shell was found bridging the segmental defect that united the host bones. Biomechanical testing showed that C9-coated allografts displayed torsional strength and stiffness equivalent to intact femurs at 6 weeks and superior to live isografts at 9 weeks. Volumetric and histomorphometric micro-computed tomography analyses demonstrated a 2-fold increase in new bone formation around C9-coated allografts, which resulted in a substantial increase in polar moment of inertia (pMOI) due to the formation of new cortical shell around the allografts. Positive correlations between biomechanics and new bone volume and pMOI were found, suggesting that the biomechanical function of the grafted femur relates to both morphological parameters. C9-coated allograft also exhibited slower resorption of the graft cortex at 9 weeks than live isograft. Both new bone formation and the persistent allograft likely contributed to the improved biomechanics of C9-coated allograft. Taken together, we propose a novel strategy to combine structural bone allograft with genetically engineered mesenchymal stem cells as a novel platform for bone tissue engineering.

Org. modification of sol-gel catalytic glassy electrodes made of a thin layer of organosilica doped with nitroxyl radical TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) crucially enhances stability in the waste-free oxidn. of alcs. to carbonyls in H2O. Structural comparison between analogous films made of organosilica and unmodified SiO2 shows that the origin of the pronounced stable activity of the ORMOSIL film lies in high hydrophobic and also in the pronounced low degree of hydrophilicity. [on SciFinder(R)]

A H2O-in-oil microemulsion was further dispersed in an aq. phase contg. Pluronic F127 as a steric stabilizer, to form a specific type of double emulsion termed emulsified microemulsion (EME). The inner microemulsion phase was made from glycerol-monooleate (GMO), R(+)-limonene, EtOH and glycerol. SAXS (small x-ray scattering), PGSE-NMR (pulse gradient spin echo-NMR), elec. cond., and cryo-TEM (cryogenic-TEM) imaging techniques were used to confirm the existence of inner W/O nano-droplets after 2nd emulsification step and upon EME aging. Spherical globules of EME without long-range internal order were obsd. by the SAXS measurements and the cryo-TEM images. The av. globule size of ∼200-300 nm remained intact for at least 6 mo. [on SciFinder(R)]

Analysis of extreme hydrometeorological events is important for characterizing and better understanding the meteorological conditions that can generate severe rainstorms and the consequent catastrophic flooding. According to several studies (e.g., Alpert et al., 2004; Wittenberg et al., 2007), the occurrence of such extreme events is increasing over the eastern Mediterranean although total rain amounts are generally decreasing. The current study presents an analysis of an extreme event utilizing different methodologies: (a) synoptic maps and high resolution satellite imagery for atmospheric condition analysis; (b) rainfall analysis by rain gauges data; (c) meteorological radar rainfall calibration and analysis; (d) field measurements for estimating maximum peak discharges; and, (e) high resolution aerial photographs together with field surveying for quantifying the geomorphic impacts. The unusual storm occurred over Israel between 30 March and 2 April, 2006. Heavy rainfall produced more than 100mm in some locations in only few hours and more than 200mm in the major core area. Extreme rain intensities with recurrence intervals of more than 100 years were found for durations of 1 h and more as well as for the daily rain depth values. In the most severely affected area,Wadi Ara, extreme flash floods caused damages and casualties. Specific peak discharges were as high as 10–30m3/s/km2 for catchments of the size of 1–10 km2, values larger than any recorded floods in similar climatic regions in Israel.